Skip to main content
SpringerLink
Log in

Untersuchungen über eine lipolytisch wirksame Substanz aus Schweinehypophysen (Fraktion H)

Extraktion und Reinigung der lipolytischen Fraktion

Investigations about a lipolytic substance isolated from pig pituitaries (Fraction H)

Extraction and purification of the substance

  • Published:
Zeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie

Summary

The extraction and further purification of a lipolytic substance from hog pituitaries (Fraction H) are reported. This substance has physico-chemical properties identical with Fraction H extracted from pituitaries of pigs byRudman et al. Further purification was achieved by Sephadex-chromatographie. The lipolytic activity in the rabbit was augmented in vitro to 0.1 Μg/ml and in vivo to 63 Μg as minimal effective dose. A contamination with ACTH could be excluded by chromatography on Sephadex G 50.

Zusammenfassung

Es wird über die Gewinnung und weitere säulenchromatographische Reinigung einer lipolytisch wirksamen Substanz aus Schweinehypophysen (Fraktion H) berichtet. Auf Grund der untersuchten physikalisch-chemischen Eigenschaften kann gesagt werden, daß diese Substanz mit der vonRudman et al. extrahierten Fraktion H identisch ist. Durch Chromatographie an Sephadex G 50 konnte die Fraktion weiter gereinigt werden; die lipolytische Aktivität pro Gewichtseinheit wurde dadurch von 0,3 Μg auf 0,1 Μg in vitro bzw. von 125 Μg auf 63 Μg in vivo als minimal wirksame Dosen gesteigert, wie Untersuchungen an Meerschweinchen und Kaninchen ergaben. Eine Kontamination mit lipolytisch wirksamen Mengen ACTH wurde durch säulenehromatographische Untersuchungen auf Sephadex G 50 weitgehend ausgeschlossen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Di Girolamo, M., D. Rudman, M. B. Reid, andF. Seidman: Effect of pituitary upon serum free fatty acid concentration of the rabbit. Endocrinology68, 457 (1961).

    Google Scholar 

  2. Dole, V. P., andH. Meinertz: Microdetermination of long-chain fatty acids in plasma and tissue. J. biol. Chem.235, 2595 (1960).

    PubMed  CAS  Google Scholar 

  3. Gordon, R. S.: Unesterified fatty acids in human blood plasma. II. The transport function of unesterifled fatty acids. J. clin. Invest.36, 810 (1957).

    Article  PubMed  CAS  Google Scholar 

  4. Grassmann, W., u.K. Hannig: Ein einfaches Verfahren zur Analyse der Serumproteine und anderer Proteingemische. Naturwissenschaften37, 496 (1950).

    Article  CAS  Google Scholar 

  5. Hirs, C. H. W., St. Moore, andW. H. Stein: A Chromatographic investigation on pancreatic ribonuclease. J. biol. Chem.200, 493 (1953).

    PubMed  CAS  Google Scholar 

  6. Hirsch, R. L., D. Rudman, R. Ireland, andR. K. Skaly: Movement of free fatty acids into and out of the blood stream in normal rabbits and in rabbits injected subcutanously with the pituitary adipokinetic substances Fraction H and adrenocorticotropin. J. Lipid Res.4, 289 (1963).

    PubMed  CAS  Google Scholar 

  7. Hirsch, R. L., D. Rudman, andR. Travers: Movement of lipids into and out of blood during hyperlipemia induced in rabbits by pituitary extract and Fraction H. J. Lipid Res.7, 182 (1966).

    PubMed  CAS  Google Scholar 

  8. Rudman, D.: Adipokinetic actions of adrenocorticotropin, thyroidstimulating hormone, vasopressin, alpha- and beta-melanocytestimulating hormones, fraction H, epinephrine and norepinephrine in the rabbit, guinea pig, hamster, rat, pig and dog. Endocrinology72, 527 (1963).

    CAS  Google Scholar 

  9. —: The adipokinetic action of polypeptide and amine hormones upon adipose tissue of various animal species. J. Lipid Res.4, 119 (1963).

    PubMed  CAS  Google Scholar 

  10. —: The adipokinetic properties of hypophyseal peptides. Ergebn. Physiol.56, 298 (1965).

    Google Scholar 

  11. —,M. Di Girolamo, M. F. Malkin, andL. A. Garcia: The adipokinetic properties of hypophyseal peptides and catecholamines: a problem in comparative endocrinology. In: Handbook of Physiology, Section V, Adipose Tissue, p. 533. Ed.E. A. Renold, andC. F. Cahill. Amer. Physiol. Soc., Washington 1965.

    Google Scholar 

  12. —,R. L. Hirsch, F. E. Kendall, andSt. J. Brown: An adipokinetic component of the pituitary gland: Purification, physical, chemical and biologic properties. Recent. Progr. Hormone Res.18, 89 (1962).

    Google Scholar 

  13. —,M. B. Reid, F. Seidman, M. Di Girolamo, F. Wertheim, andS. Bern: Purification and properties of a component of the pituitary gland which produces lipemia in the rabbit. Endocrinology68, 273 (1961).

    PubMed  CAS  Google Scholar 

  14. —,F. Seidman, St. J. Brown, andR. L. Hirsch: Adipokinetic activity of porcine fraction H in the rabbit, guinea pig, rat and mouse. Endocrinology70, 233 (1962).

    Article  PubMed  CAS  Google Scholar 

  15. — —, andM. B. Reid: Preparation of a lipemia-producing fraction from hog pituitary glands. J. clin. Invest.38, 1038 (1959).

    Google Scholar 

  16. — — —: Lipemia-producing activity of pituitary gland: Separation of a lipemia-producing component from other pituitary hormones. Proc. Soc. exp. Biol. (N.Y.)103, 315 (1960).

    CAS  Google Scholar 

  17. Schwandt, P., M. Knedel u.R. Lindlbauer: Experimentelle Untersuchungen zum Verfahren der in vitro Lipolyse. Z. klin. Chem. u. klin. Biochem.6, 81 (1968).

    CAS  Google Scholar 

  18. —, S.Werner u. H. J.Karl: In Vorbereitung.

  19. Weil, R.: Pituitary growth hormone and intermediary metabolism. Acta endow. (Kbh.) Suppl.98 (1965).

Download references

Author information

Authors and Affiliations

  1. I. Medizinische Klinik der Universität München, Deutschland

    P. Schwandt, H. J. Karl, J. Thüner & M. Knedel

Authors
  1. P. Schwandt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. J. Karl
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Thüner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Knedel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schwandt, P., Karl, H.J., Thüner, J. et al. Untersuchungen über eine lipolytisch wirksame Substanz aus Schweinehypophysen (Fraktion H). Z. Gesamte Exp. Med. 147, 246–252 (1968). https://doi.org/10.1007/BF02044608

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02044608

Key-words

Schlüsselwörter

Search

Navigation