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Effect of ethane-1-hydroxy-1,1-diphosphonate (EHDP) administration on duodenal calcium transport

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Abstract

Ethane-1-hydroxy-1,1-diphosphonate (EHDP) given subcutaneously to rats in high doses (10 mg P/Kg body weight daily×10) inhibited the transport of calcium by rat duodenal gut sacs, when45Ca serosal/mucosal concentration ratios were used to measure active transport. Electron microscopic studies revealed the accumulation of electron-dense granules in the microvilli, representing a bound form of calcium and a paucity of such granules in mitochondria, changes identical to those seen in rachitic rats. These results suggest that the defect is mediated in part by interference in transport of calcium from microvilli to other intracellular sites.

Résumé

De l'éthane-1-hydroxy-1,1-diphosphonate (EHDP), administré par voie sous-cutanée à des rats à doses élevées (10 mg P/Kg de poids par jour×10), inhibe le transport du calcium au niveau du duodenum de rats, en utilisant des rapports de concentration serum/muqueuse en45Ca pour mesurer le transfert actif. Des études de microscopie électronique montrent l'accumulation de granules denses aux électrons dans les microvillosités: ces granules seraient constitués par une forme de calcium lié. On note aussi la rareté de ces granules dans les mitochondries; ces modifications sont analogues à celles observées dans les rats rachitiques. Il semble que les phénomènes pathologiques soient liées à un trouble du transport du calcium des microvillositées vers d'autres sites intracellulaires.

Zusammenfassung

Es zeigte sich, daß bei Ratten, die mit hohen Dosen (10 mg P/kg Körpergewicht täglich×10) von Aethan-1-hydroxy-1,1-diphosphonat (EHDP) subkutan behandelt wurden, der Calciumtransport im Duodenum (gut sacs) gehemmt wurde; zur Messung des aktiven Transportes wurde das45Ca-Konzentrationsverhältnis Serosa/Mucosa benützt. Elektronenmikroskopische Untersuchungen zeigten in den Microvilli eine Anhäufung von elektronendichten Granula, die eine gebundene Form von Calcium darstellen; eine kleine Anzahl solcher Granula fand sich in den Mitochondrien; diese Veränderungen können auch bei rachitischen Ratten beobachtet werden. Aus den Resultaten kann geschlossen werden, daß dieser Defekt teilweise durch eine Interferenz des Calciumtransportes von den Microvilli zu anderen intrazellulären Stellen bedingt ist.

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Authors and Affiliations

  1. Department of Medicine, University of Vermont, College of Medicine, 05401, Burlington, Vermont, USA

    E. L. Krawitt M. D., H. W. Sampson, A. S. Kunin & J. L. Matthews

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  1. E. L. Krawitt M. D.
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  2. H. W. Sampson
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  3. A. S. Kunin
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  4. J. L. Matthews
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Baylor College of Dentistry

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Krawitt, E.L., Sampson, H.W., Kunin, A.S. et al. Effect of ethane-1-hydroxy-1,1-diphosphonate (EHDP) administration on duodenal calcium transport. Calc. Tis Res. 15, 21–29 (1974). https://doi.org/10.1007/BF02059040

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