Summary
One hind leg of 80 adult rats of the Sprague-Dawley strain was made osteoporotic by immobilization for 9 weeks. Osteoporosis was noted in both the femur and the tibia when the hydrated gross bone density and the bone surface areas were measured. No signs of reversibility were observed during 10 weeks after the period of immobilization. Tetracycline and DCAF labelling failed to show significant signs of increased bone formation during the 10 weeks after remobilization. At the moment of remobilization and for some weeks thereafter, there were signs of depressed mitotic activity in the bone cells when expressed as the3H-thymidine/DNA ratio. The conclusion was that neither the cell-proliferation rate nor the cellular activity increases sufficiently for restitution of the disuse osteoporosis.
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Hinegardner, R.I.: An improved fluorometric assay for DNA. Anal. Biochem.39, 197–201 (1971)
Hughes, W.L.: The metabolic stability and deoxyribonucleic acid. In: Kinetics of cellular proliferation (F. Stohlman, jr., ed.), p. 83. New York and London: Grune and Stratton 1959
Hughes, W.L.: Cellular proliferation in the mouse as revealed by autoradiography with tritiated thymidine. Proc. Nat. Academ. Sci.44, 476 (1958)
Jones, I., Lemperg, R.: Determination of DNA in bone tissue. In preparation (1976)
Kember, N.F.: Cell division in enchondral ossification. J. Bone Jt Surg.B-42, 824–839 (1960)
Kissane, J.M., Robins, E.: The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J. Biol. Chem.233, 184–188 (1958)
Lindgren, J.U.: The effect of thyroparathyroidectomy on the development of disuse osteoporosis in adult rats. Clin. Orthop. Rel. Res.118, 251–256 (1976)
Mattson, S.: The reversibility of disuse osteoporosis. Experimental studies in the adult rat. Acta orthop. scand. suppl. No.144 (1972)
Mueller, K., Trias, A., Ray, R.D.: Bone density and composition. J. Bone Jt Surg.48-A, 138–148 (1966)
Nilsson, B.E.: Post-traumatic osteopenia. Acta orthop. scand. suppl. No.91 (1966)
Snedecor, G.W., Cochran, W.C.: Statistical methods. Ames: Iowa State University Press 1967
Telhag, H., Gudmundson, C.: Nucleic acids in degenerative joint disease. Clin. Orthop.88, 247–251 (1972)
Wagner, H.: Präsenile Osteoporose. Physiologie des Knochenumbaus und Messung der Spongiosadichte. Stuttgart: Georg Thieme 1965
Westlin, N.E.: Loss of bone mineral after Colles' fracture. Clin. Orthop. Rel. Res.102, 194–199 (1974)
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Lindgren, U., Mattsson, S. The reversibility of disuse osteoporosis. Calc. Tis Res. 23, 179–184 (1977). https://doi.org/10.1007/BF02012784
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DOI: https://doi.org/10.1007/BF02012784