Summary
The influence of estradiol and progesterone, alone or in combination, on the discrete phases of matrix-induced endochondral bone formation was investigated. Administration of estradiol and progesterone in combination increased mesenchymal cell proliferation, as indicated by [3H] thymidine incorporaton into acid precipitable material. However, ornithine decarboxylase (ODC) activity was significantly suppressed by the combination of estradiol and progesterone. Also, this treatment did not influence the35SO4 incorporation into proteoglycans on day 7. Mineralization of newly induced bone was quantitated by alkaline phosphatase,45Ca incorporation into bone mineral and calcium content, and was found to be significantly increased by progesterone alone and in combination with estradiol in both matrix-induced plaques and tibial metaphysis. These results demonstrated the stimulatory role of progesterone in combination with estradiol in bone formation and mineralization.
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References
Tonna EA (1973) Hormonal influence on skeletal growth and regeneration. In: Lobue J, Gordon A. S. (eds) Humoral control of growth and differentiation. Academic Press, New York-London, pp. 275–359
Albright F, Smith PH, Richardson AM (1941) Postmenopausal osteoporosis: Its clinical features. JAMA 116:2465–2474
Albright F, Reinfenstein EC Jr (1948) The parathyroid glands and metabolic bone disease. Williams and Wilkens, Baltimore, pp 145–201.
Heaney RP, Recker RR, Saville PD (1975) Estrogen effects on bone remodeling in menopause (abstract). Clin Res 23:535A
Meema S, Bunker ML, Meema HE (1975) Preventive effect of estrogen on postmenopausal bone loss. Arch Intern Med 135:1436–1440
Lindsay R, Hart DM, Aitkin JM, MacDonald EB, Anderson JB, Clarke AC (1976) Long-term prevention of postmenopausal osteoporosis by estrogen. Evidence of an increased bone mass after delayed onset of estrogen treatment. Lancet 1:1038–1041
Hernberg CA (1960) Treatment of postmenopausal osteoporosis with oestrogens and androgens. Acta Endocrinol (Kbh) 34:51–59
Shapiro JR, Moore WT, Jorgennsen H, Reid J, Epps CH, Whedon D (1975) Osteoporosis. Arch Intern Med 135:563–567
Cruess RL, Hong KC (1979) The effect of long-term estrogen administration on bone metabolism in the female rat. Endocrinology 104:1188–1193
Heaney RP (1969) Estrogen effects on the skeleton. In: Salhanick HA, Kipnis DM, Vande Wiele RL, (eds) Metabolic effects of gonadal hormones and contraceptive steroids. Plenum Press, New York-London, pp 493–502
Raisz LG, Bingham PJ (1972) Effect of hormones on bone development. Ann Rev Pharmacol 12:337–352
Rasmussen H, Bordier P (1974) The physiological and cellular basis of metabolic bone disease. Williams and Wilkens, Baltimore, pp 200–275
Langeland N (1975)In vitro studies on collagen metabolism in metaphyseal rat bone. Acta Endocrinol 80:775–783
Caputo CB, Meadows D, Raisz LG (1976) Failure of estrogens and androgens to inhibit bone resorption in tissue culture. Endocrinology 98:1065–1068
Liskova M (1976) Influence of estrogens on bone resorption in organ culture. Calcif Tissue Res 22:207–218
Nordin BEC, Young MM, Bulusu L, Horsman A (1970) In: Barzel US (ed) Osteoporosis. Grune and Stratton, New York, pp 47–67
Riggs BL, Jowsey J, Kelly PJ, Jones JD, Maher FT (1969) Effect of sex hormones on bone in primary osteoporosis. J Clin Invest 48:1065–1072
Orimo H, Fujita T, Yoshikawa M (1972) Increased sensitivity of bone to parathyroid hormone in ovariectomized rats. Endocrinology 90:760–763
Heaney RP (1965) A unified concept of osteoporosis. Am J Med 39:877–880
Horsman A, Gallagher JC, Simpson M, Nordin BEC (1977) Prospective trial on estrogen and calcium in postmenopausal women. Br Med J 2:789–792
Lindsey R, Aitkin JM, Anderson JB, Hart DM, MacDonald EB, Clarke AC. (1976) Long-term prevention of postmenopausal osteoporosis by estrogen. Lancet 1:1038–1040
Meema HE, Meema S (1968) Prevention of postmenopausal osteoporosis by hormone treatment of the menopause. Can Med Assoc J 99:248–251
Katz FH (1968) Effects of estradiol and estriol on the deposition of injected radioactive proline, hydroxyproline and tyrosine in man. Acta Endocrinol 58:644–672
Katz FH, Kappas A. (1968) Influence of estradiol and estriol on urinary excretion of hydroxyproline in man. J Lab Clin Med 71:65–74
Simmons DJ (1976) Comparative physiology of bone. In: Bourne GH (ed) The biochemistry and physiology of bone, Vol. 4, Academic Press, New York, pp 445–516
Aitken JM, Armstrong E, Anderson JB (1972) Osteoporosis after oophorectomy in the mature female rat and the effect of oestrogen and/or progestogen replacement therapy in its prevention. J Endocrinol 55:79–87
Langeland N, Teig V (1975)In vitro studies on collagen metabolism in metaphyseal rat bone. B. The early effects of estradiol-17β, Acta Endocrinol 80:784–794
Reddi AH, Anderson WA (1976) Collagenous bone matrix-induced endochondral ossification and hemopoiesis. J Cell Biol 69:557–572
Reddi AH, Huggins CB (1972) Biochemical sequences in the transformation of normal fibroblast in adolescent rat. Proc Natl Acad Sci USA 69:1601–1605
Reddi AH, Huggins CB (1975) The formation of bone marrow in fibroblast transformation ossicles. Proc Natl Acad Sci USA 72:2212–2216
Rath NC, Reddi AH (1978) Changes in ornithine decarboxylase activity during matrix-induced cartilage, bone and bone marrow differentiation. Biochem Biophys Res Commun 81:106–113
Reddi AH, Hascall VC, Hascall GK (1978) Changes in proteoglycan types during matrix-induced cartilage and bone development. J Biol Chem 253:2429–2436
Weiss RE, Reddi AH (1980) Influence of experimental diabetes and insulin on matrix-induced cartilage and bone differentiation. American Physiological Society E200–E207
Burton KA (1956) A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323
Janne J, Williams-Ashman HG (1971) On the purification of L-ornithine decarboxylase from rat prostate and effect of thiol compounds on the enzyme. J Biol Chem 246:1725–1732
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Reddi AH, Sullivan N (1980) Matrix-induced endochondral bone differentiation: Influence of hypophysectomy, growth hormone, and thyroid stimulating hormone. Endocrinology 107:1291–1299
Willis JB (1960) The determination of metal in blood serum by atomic absorption spectroscopy. I. Calcium. Spectrochim Acta 16:259–272
Chen PS, Toribara TY, Warner H (1956) Microdetermination of phosphorous. Anal Chem 28:1756–1758
Cruess RL, Hong KC (1979) The effect of long-term estrogen administration on bone metabolism in the female rat. Endocrinology 104:1188–1193
Tabor CW, Tabor H (1976) 1,4 Diaminobutane (putrescine) spermidine and spermine, Ann Rev Biochem 45:285–306
Rath NC, Reddi AH (1979) Influence of adrenalectomy and dexamethasome on matrix-induced endochondral bone differentiation. Endocrinology 104:1968–1704
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Burnett, C.C., Reddi, A.H. Influence of estrogen and progesterone on matrix-induced endochondral bone formation. Calcif Tissue Int 35, 609–614 (1983). https://doi.org/10.1007/BF02405102
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DOI: https://doi.org/10.1007/BF02405102