Summary
During Sendai virus-mediated fusion of mouse ascites cells, there is a loss of intracellular metabolites; at the same time accumulation from the medium is inhibited. This failure to maintain selective permeability does not occur at 0°C; it is unaffected by cytochalasin B, which inhibits fusion. It therefore represents a discrete, temperature-dependent event and may result from a weakening in membrane architecture necessary to achieve fusion.
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Ahkong, Q. F., Cramp, F. C., Fisher, D., Howell, J. I., Tampion, W., Verrinder, M., Lucy, J. A. 1973. Chemically-induced and thermally-induced cell fusion: Lipid-lipid interactions.Nature, New Biol. 242:215
Beck, J. S., Jay, A. W. L., Saari, J. T. 1972. Effects of cytochalasin B on osmotic fragility and deformability of human erythrocytes.Canad. J. Physiol. Pharmacol. 50:684
Blazyk, J. F., Steim, J. M. 1972. Phase transitions in mammalian membranes.Biochim. Biophys. Acta 266:737
Cassells, A. C. 1973. The release of lactate dehydrogenase from chick embryo cells infected with Semliki Forest virus.J. Gen. Virol. 18:203
Davis, A. T., Estensen, R., Quie, P. G. 1971. Cytochalasin B. III. Inhibition of human polymorphonuclear leukocyte phagocytosis.Proc. Soc. Exp. Biol., N.Y. 137:161
Dulbecco, R., Vogt, M. 1954. Plaque formation and isolation of pure lines with poliomyelitis virus.J. Exp. Med. 99:167
Estensen, R. D. 1971. Cytochalasin B. I. Effect on cytokinesis of Novikoff hepatoma cells.Proc. Soc. Exp. Biol., N.Y. 136:1256
Estensen, R. D., Plagemann, P. G. W. 1972. Cytochalasin B. Inhibition of glucose and glucosamine transport.Proc. Nat. Acad. Sci. 69:1430
Frye, L. D., Edidin, M. 1970. The rapid intermixing of cell surface antigens after the formation of mouse-human heterokaryons.J. Cell Sci. 7:319
Gilbert, V. E. 1963. Enzyme release from tissue cultures as an indicator of cellular injury by viruses.Virology 21:609
Harris, H. 1970. Cell Fusion. Clarendon Press, Oxford, p. 7
Kletzien, R. F., Perdue, J. F. 1973. The inhibition of sugar transport in chick embryo fibroblasts by cytochalasin B.J. Biol. Chem. 248:711
Kletzien, R. F., Perdue, J. F., Springer, A. 1972. Cytochalasin A and B. Inhibition of sugar uptake in cultured cells.J. Biol. Chem. 247:2964
Knox, P., Pasternak, C. A. 1973. Serum-mediated membrane changes.Biochem. Soc. Trans. 1:430
Lachmann, P. J., Bowyer, D. E., Nicol, P., Dawson, R. M. C., Munn, E. A. 1973. Studies on the terminal stages of complement lysis.Immunology 24:135
Lucy, J. A. 1970. The fusion of biological membranes.Nature 227:815
Maslow, D. E., Mayhew, E. 1972. Cytochalasin B prevents specific sorting of reaggregating embryonic cells.Science 177:281
Orr, T. S. C., Hall, D. E., Allison, A. C. 1972. Role of contractile microfilaments in the release of histamine from mast cells.Nature 236:350
Pasternak, C. A., Knox, P. 1973. Phospholipid turnover as a determinant of membrane function.In: Membrane Mediated Information. P. W. Kent, editor. Medical and Technical Publishing Co. Ltd., Lancaster, (In press)
Pasternak, C. A., Quinn, P. J. 1974. Structural consequences of phospholipid turnover.Biochim. Biophys. Acta (Membrane Reviews). (in press)
Plagemann, P. G. W. 1971. Choline metabolism and membrane formation in rat hepatoma cells grown in suspension culture. III. Choline transport and uptake by simple diffusion and lack of direct exchange with phosphatidylcholine.J. Lipid Res. 12:715
Poste, G. 1972. Mechanisms of virus-induced cell fusion.Int. Rev. Cytol. 33:157
Poste, G., Allison, A. C. 1971. Membrane fusion reaction: A theory.J. Theoret. Biol. 32:165
Rash, J. E., Fambrough, D. 1973. Ultrastructural and electrophysiological correlates of cell coupling and cytoplasmic fusion during myogenesis in vitro.Devel. Biol. 30:166
Renner, E. D., Plagemann, P. G. W., Bernlohr, W. 1972. Permeation of glucose by simple and facilitated diffusion by Novikoff rat hepatoma cells in suspension culture and its relationship to glucose metabolism.J. Biol. Chem. 247:5765
Reporter, M., Norris, G. 1973. Reversible effects of lysolecithin on fusion of cultured rat muscle cells.Differentiation 1:83
Rotman, B., Papermaster, B. W. 1966. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.Proc. Nat. Acad. Sci. 55:134
Sanger, J. W., Holtzer, H. 1972. Cytochalasin B: Effects on cell morphology, cell adhesion, and mucopolysaccharide synthesis.Proc. Nat. Acad. Sci. 69:253
Taylor, R. B., Duffus, W. P. H., Raff, M. C., de Petris, S. 1971. Redistribution and pinocytosis of lymphocyte surface immunoglobulin molecules induced by anti-immunoglobulin antibody.Nature, New Biol. 233:225
Toister, Z., Loyter, A. 1973. The mechanism of cell fusion. II. Formation of chicken erythrocyte polykaryons.J. Biol. Chem. 248:422
Wagner, R., Rosenberg, M., Estensen, R. 1971. Endocytosis in Chang liver cells.J. Cell. Biol. 50:804
Welshons, W. J., Gibson, B. H., Scandlyn, B. J. 1962. Slide processing for the examination of male mammalian meiotic chromosomes.Stain Technol. 37:1
Wessels, N. K., Spooner, B. S., Ash, J. F., Bradley, M. O., Luduena, M. A., Taylor, E. L., Wrenn, J. T., Yamada, K. M. 1971. Microfilaments in cellular and developmental processes.Science 171:135
Williams, J. A., Wolff, J. 1971. Cytochalasin B inhibits thyroid secretion.Biochem. Biophys. Res. Commun. 44:422
Yoshinaga, M., Waksman, B. H., Malawista, S. E. 1972. cytochalasin B inhibits lymphotoxin production by antigen-stimulated lymphocytes.Science 176:1147
Zigmond, S. H., Hirsch, J. G. 1972. Cytochalasin B: Inhibition ofd-2-deoxyglucose transport into leukocytes and fibroblasts.Science 176:1432
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Pasternak, C.A., Micklem, K.J. Permeability changes during cell fusion. J. Membrain Biol. 14, 293–303 (1973). https://doi.org/10.1007/BF01868082
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DOI: https://doi.org/10.1007/BF01868082