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Protein kinase A-regulated Cl− channel in ML-1 human hematopoietic myeloblasts

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Abstract

Using the inside-out patch clamp technique, we identified a Cl− channel in patches from the membrane of cultured human hematopoietic myeloblastic leukemia ML-1 cells. The Cl− channel was not seen at negative membrane potentials in excised patches until the membrane potential was depolarized to greater than +40 mV. The channel was also activated by addition of cAMP-dependent protein kinase (PKA) catalytic subunit at physiological membrane potential (−40 mV). Biophysical studies of the Cl− channel revealed that the current-voltage (I-V) relationship of the Cl− channel was outwardly rectifying in symmetrical 142 mm Cl− solutions. Single channel conductances were 48 pS for the outward current measured at +60 mV and 27 pS for the inward current at −60 mV. The open time constant of the channel was dependent on the membrane potential and was significantly prolonged at positive membrane potentials. Channels activated by cAMP-dependent protein kinase spent a significantly longer time in the open state compared to those channels activated by depolarization pulses. Pharmacological properties of the Cl− channel were also studied. Two anion transport inhibitors, anthracene-9-carboxylic acid (9-AC) and 4,4-diisothiocyanatostilbene-2,2-disulfonic acid (DIDS) caused a flickering block of the channel. Half-inhibitory concentrations (IC50) for 9-AC and DIDS were 174 ± 20 and 70±16 μm, respectively. Blockade of the Cl− channel by 9-AC or DIDS was completely reversible. Our findings suggest that outwardly rectifying Cl− channels (ORCC) are present in human hematopoietic myeloblasts. The function of ORCC may be involved in hormone-regulated cell growth, cell volume regulation and immune responses.

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Authors and Affiliations

  1. Department of Physiology and Biophysics, Wright State University School of Medicine, 45435, Dayton, Ohio

    B. Xu & L. Lu

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  1. B. Xu
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  2. L. Lu
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We thank Dr. R.E. White and Ms. M.P. Nardino for reviewing the manuscript. This study was partly supported by National Institutes of Health grant GM46834 (to L.L.).

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Xu, B., Lu, L. Protein kinase A-regulated Cl− channel in ML-1 human hematopoietic myeloblasts. J. Membarin Biol. 142, 65–75 (1994). https://doi.org/10.1007/BF00233384

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  • DOI: https://doi.org/10.1007/BF00233384

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