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Relationship of alcohol-induced changes in Mg2+-ATPase activity of rabbit intestinal brush border membrane with changes in fluidity of its lipid bilayer

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Abstract

We examined the effects of seven n-alkyl alcohols (from n-butyl to n-undecyl alcohol), isoamyl alcohol and benzyl alcohol on the activity of membrane enzyme Mg2+-ATPase of the rabbit small intestinal brush border membrane. Their relationships with the changes in the fluidity of the membrane lipid bilayer were examined through studies on the fluorescence anisotropies of diphenylhexatriene (DPH) and its ionic derivatives. Good linear correlations were found both between the partition coefficients of the alcohols and their concentrations causing similar decreases in the activity of Mg2+-ATPase and between their partition coefficients and the alcohol-induced changes in fluorescence anisotropies. Within the concentration range of the alcohols tested, including isoamyl alcohol and benzyl alcohol, the decreases in activity of the membrane enzyme Mg2+-ATPase clearly corresponded with the decreases in fluorescence anisotropy of DPH, which is thought to be located within the hydrophobic core of the membrane. From these findings, one possible explanation is that inhibition of this enzyme by the alcohols is due to perturbation of the lipid bilayer of the brush border membrane.

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Authors and Affiliations

  1. Niigata College of Pharmacy, Kamishin'ei-cho 5-13-2, 950-21, Niigata, Japan

    S. Kitagawa, Y. Sugaya, M. Nishizawa & H. Hirata

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  1. S. Kitagawa
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  2. Y. Sugaya
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  3. M. Nishizawa
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  4. H. Hirata
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Additional information

The authors thank M. Takano, PhD and Y. Tomita, PhD, Department of Pharmacy, University Hospital of Kyoto University, for instruction in preparation of the brush border membrane vesicles. This work was supported in part by grants from the Japanese Ministry of Education, Science and Culture (05671795 and 06304044) and Takeda Science Foundation.

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Kitagawa, S., Sugaya, Y., Nishizawa, M. et al. Relationship of alcohol-induced changes in Mg2+-ATPase activity of rabbit intestinal brush border membrane with changes in fluidity of its lipid bilayer. J. Membarin Biol. 146, 193–199 (1995). https://doi.org/10.1007/BF00238008

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  • DOI: https://doi.org/10.1007/BF00238008

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