Abstract
The cRNA for Torpedo californica Na+/K+-ATPase β-subunit (cRNAβ) was injected into Xenopus oocytes alone or with the cRNA for the Na+/K+-ATPase α-subunit (cRNAα). When cRNAβ was injected alone, the amount of the β-subunit that accumulated in oocytes increased with increasing amounts of injected cRNAβ. When cRNAβ and cRNAα were injected simultaneously, less β-subunit accumulated than when cRNAβ was injected alone, whereas the Na+/K+-ATPase activity increased markedly. The decrease in the accumulation of the β-subunit was dose-dependent upon the cRNAα. The mutant β-subunit unable to assemble with the α-subunit accumulated in oocytes independently of cRNAα, suggesting that post-translational control mechanisms may serve to reduce the accumulation of the β-subunit.
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This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (No. 05259226, No. 06454149).
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Ueno, S., Takeda, K., Noguchi, S. et al. The expression of Na+/K+-ATPase β-subunit cRNA injected into Xenopus oocytes is affected by coinjection with α-subunit cRNA. J. Membarin Biol. 148, 51–56 (1995). https://doi.org/10.1007/BF00234155
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DOI: https://doi.org/10.1007/BF00234155