Abstract
Methods are described for the observation, enrichment and isolation (from various freshwater samples) of bacteria of the generaPlanctomyces andPirella. Because immature buds were easily dislodged by shearing forces, slide culture techniques and direct microscopy of the budding process are recommended. An “auxanographic” technique to detect possible stimulation by soluble substrates was based on the diffusion of these substrates from peripherally placed crystals into the agar of a slide culture. Nearly every water sample investigated contained representatives of the generaPlanctomyces, Pirella, andBlastobacter, as well as budding cocci. Six enrichment techniques were tried; some enrichment experiments lasted several months. Allowing samples without added substrate to stand for a long time or generally employing nutrient-poor media were most successful. The “petri dish method,” taking advantage of attachment of many budding bacteria to glass surfaces, was especially useful for increasing the numbers ofPlanctomyces spp. Pure cultures obtained from freshwater samples were tentatively placed in the generaPlanctomyces, Pirella, andBlastobacter. One strain appeared to represent a new genus of gram-positive, budding, and nonprosthecate bacteria.
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References
Bauld J, Staley JT (1976)Planctomyces maris sp. nov.: a marine isolate of thePlanctomyces-Blastocaulis group of budding bacteria. J Gen Microbiol 97:45–55
Beijerinck MW (1888) Auxanography, a method useful in microbial research, involving diffusion in gelatin. Arch Nederl des Sciences Exactes et Naturelles (Haarlem) 23:367–372
Geitler L (1975)Chamaesiphonosira cymbellicola n. gen., n. sp., ein knospenbildendes Bakterium und sein spezialisierter Epiphytismus. Arch Microbiol 103:231–236
Gossling J, Moore WEC (1975)Gemmiger formicilis n. gen., sp., an anaerobic budding bacterium from intestines. Int J Syst Bacteriol 25:202–207
Haidu L (1974) Observations of the bacterium speciesPlanctomyces guttaeformis Hortobagyi (Caulobacteriales). Arch Hydrobiol 74:172–185
Henrici AT, Johnson DE (1935) Studies of freshwater bacteria. II. Stalked bacteria, a new order of Schizomycetes. J Bacteriol 30:61–85
Hirsch P (1972) Two identical genera of budding and stalked bacteria:Planctomyces Gimesi 1924 andBlastocaulis Henrici and Johnson 1935. Int J Syst Bacteriol 22:107–111
Hirsch P (1972) Neue Methoden zur Beobachtung und Isolierung ungewöhnlicher oder wenig bekannter Wasserbakterien. Zeit f Allgem Mikrobiol 12:203–218
Hirsch P (1974) Budding bacteria. Ann Rev Microbiol 28:391–444
Hirsch P (1980) Distribution and pure culture studies of morphologically distinct Solar Lake microorganisms. In: A Nissenbaum (ed) Hypersaline brines and evaporitic environments. Chapt 4. Elsevier Sci Publ Amsterdam, pp 41–60
Hirsch P, Conti SF (1964) Biology of budding bacteria. I. Enrichment, isolation and morphology ofHyphomicrobium spp. Arch Mikrobiol 48:339–357
Hirsch P, Müller M, Schlesner H (1977) New aquatic budding and prosthecate bacteria and their taxonomic position. In: Skinner FA, Shewan JM (eds). Aquatic microbiology. Proc Symp Lancaster UK, Appl Bacteriol Symp Ser No 6, 107–133, Academic Press, London
Hirsch P, Rades-Rohkohl E (1983) Die Zusammensetzung der natürlichen GrundwasserMikroflora und Untersuchungen über ihre Wechselbeziehungen mit Fäkalbakterien. DVGW Schriftenreihe Wasser Eschborn 35:59–80
Hirsch P, Rheinheimer G (1968) Biology of budding bacteria. V. Budding bacteria in aquatic habitats: occurrence, enrichments and isolation. Arch Mikrobiol 62:289–306
Hirsch P, Zavarzin GA (1974) GenusBlastobacter Zavarzin 1961. In: Buchanan RE, Gibbons NE (eds). Bergey's manual of determinative bacteriology, 8th ed, Williams and Wilkins, Baltimore, p 159
Hortobagyi T (1965) NeuePlanctomyces-Arten. Botaniki Közlemenyek 52:111–119
König E, Schlesner H, Hirsch P (1984) Cell wall studies on budding bacteria of thePlanctomyces/Pasteuria group and onProsthecomicrobium sp. Arch Microbiol 138:200–205
Metchnikoff ME (1888)Pasteuria ramosa. A representative of the bacteria dividing longitudinally. Ann l'Inst Pasteur 2:165–170
Moore RL (1981) The biology ofHyphomicrobium and other prosthecate budding bacteria. Ann Rev Microbiol 35:567–594
Poindexter JS (1984) Role of prostheca development in oligotrophic aquatic bacteria. In: Klug MI, Reddy CA (eds) Current perspectives in microbial ecology. Proc 3 Int Symp Microb Ecol, American Soc Microbiol Washington, pp 33–40
Sayre RM, Gherna RL, Wergin WP (1983) Morphological and taxonomic reevaluation ofPasteuria ramosa Metchnikoff 1888 and “Bacillus penetrans” Mankau 1975. Int J Syst Bacteriol 33:636–649
Schlesner H, Hirsch P (1984) Assignment of ATCC 27 377 toPirella gen. nov. asPirella staleyi comb. nov. Int J Syst Bacteriol 34:492–495
Schmidt JM, Starr MP (1978) Morphological diversity of freshwater bacteria belonging to theBlastocaulis-Planctomyces group as observed in natural populations and enrichments. Curr Microbiol 1:325–330
Stackebrandt E, Ludwig W, Schubert W, Klink F, Schlesner H, Roggentin T, Hirsch P (1984) Molecular genetic evidence for early evolutionary origin of budding peptidoglycan-less eubacteria. Nature 307:735–737
Staley JT (1973) Budding bacteria of thePasteuria-Blastobacter group. Can J Microbiol 19:606–614
Staley JT (1968)Prosthecomicrobium andAncalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
Staley JT, Marshall KC, Skerman VBD (1980) Budding and prosthecate bacteria from freshwater habitats of various trophic states. Microb Ecol 5:245–251
Vasil'eva LV, Lafitskaya TN, Namsaraev BB (1979)Angulomicrobium tetraedrale, a new genus of budding bacteria with radial cell symmetry. Microbiology Translat 48:843–849
Whittenbury R, Nicoll JM (1971) A new, mushroom-shaped budding bacterium. J Gen Microbiol 66:123–126
Hirsch P, Müller M (1985)Planctomyces limnophilus sp. nov., a stalked and budding bacterium from freshwater. System Appl Microbiol 6:276–280
Hirsch P, Müller M (1985)Blastobacter aggregatus sp. nov.,Blastobacter capsulatus sp. nov., andBlastobacter denitrificans sp. nov., new budding bacteria from freshwater habitats. System Appl Microbiol 6:281–286
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Hirsch, P., Müller, M. Methods and sources for the enrichment and isolation of budding, nonprosthecate bacteria from freshwater. Microb Ecol 12, 331–341 (1986). https://doi.org/10.1007/BF02098574
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DOI: https://doi.org/10.1007/BF02098574