Abstract
Azotobacter vinelandii was mutagenized with ethyl methanesulfonate, and colonies that did not produce the fluorescent yellow-green pigment that is characteristic of the wild type were selected. All 32 stable nonfluorescent mutants failed to secrete the siderophore azotobactin and were also impaired to some extent in the production of the second majorA. vinelandii siderophore, azotochelin. Mutants also showed differences in their capacity to grow on medium supplemented with either 200 μM bipyridyl or 200 μM Fe (III). In the absence of iron, an 84-kilodalton outer membrane protein, which is a major derepressed component, was missing in some of the mutants. Thus, siderophore production inA. vinelandii appears to be a highly integrated system in which the syntheses of azotobactin and azotochelin are functionally coupled.
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Glick, B.R., Menhart, N., Soong, N.W. et al. Isolation and partial characterization of siderophore mutants ofAzotobacter vinelandii . Current Microbiology 17, 343–346 (1988). https://doi.org/10.1007/BF01570875
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DOI: https://doi.org/10.1007/BF01570875