Abstract
TheE. coli rnc gene encodes the double-stranded, RNA-specific ribonuclease III (RNaseIII). A novel bacteriophage, gy1, was isolated, and its propagation inE. coli was shown to depend on the expression of RNaseIII in the cell. (a) gyl has a low efficiency of plating on rnc+ strains and a high efficiency of plating on a rnc− E. coli strain harboring the rnc 105 point mutation that renders its RNaseIII product inactive. (b) gy1 has a high efficiency of plating on rnc− strains in which thernc gene is disrupted by a ΔTn10 insertion. (c) Plasmids harboring a rnc+ gene that were introduced into the rnc− strains described above reduced the efficiency of plating of gy1.
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Davidov, Y., Zivion, G. & Pines, O. Ribonuclease III reduces the efficiency of bacteriophage gy1 propagation inE. coli . Current Microbiology 24, 63–66 (1992). https://doi.org/10.1007/BF01570899
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DOI: https://doi.org/10.1007/BF01570899