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Effects of fumarate,l-malate, and anAspergillus oryzae fermentation extract ond-lactate Utilization by the ruminal bacteriumSelenomonas ruminantium

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Abstract

Growth ofSelenomonas ruminantium HD4 in medium that contained 21mm d-lactate was stimulated to varying degrees by 10mm l-malate, 10mm fumarate, and 2% (v/v)Aspergillus oryzae fermentation extract (Amaferm). Amaferm treatment caused the greatest growth stimulation. Initial uptake rates (30s) and long-term uptake rates (30 min) ofd-lactate by whole cells ofS. ruminantium were increased in the presence of 10mm l-malate. Amaferm (25 μl/ml) also stimulated long-term uptake rates ofd-lactate, whereas fumarate had no effect. Initial uptake ofd-lactate was depressed in the presence of fumarate or Amaferm. When eitherl-malate, fumarate, or Amaferm was included in thed-lactate growth medium, a homosuccinate fermentation resulted and an inverse relationship was observed between growth (protein synthesis) and succinate production. Recent research demonstrated that Amaferm containsl-malate, and this dicarboxylic acid may be involved in stimulatingd-lactate utilization byS. ruminantium.

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Author notes

  1. David J. Nisbet

    Present address: Southern Plains Area Food Animal Protection Laboratory, USDA-ARS, 77845, College Station, TX, USA

Authors and Affiliations

  1. Department of Animal and Dairy Science, The University of Georgia, 308 Livestock-Poultry Building, 30602, Athens, Georgia, USA

    David J. Nisbet & Scott A. Martin

  2. Department of Microbiology, The University of Georgia, 308 Livestock-Poultry Building, 30602, Athens, Georgia, USA

    Scott A. Martin

Authors
  1. David J. Nisbet
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  2. Scott A. Martin
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Nisbet, D.J., Martin, S.A. Effects of fumarate,l-malate, and anAspergillus oryzae fermentation extract ond-lactate Utilization by the ruminal bacteriumSelenomonas ruminantium . Current Microbiology 26, 133–136 (1993). https://doi.org/10.1007/BF01577366

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