Effect of linear polarized light radiation on impaired mitochondrial oxidative phosphorylation in skeletal muscle

Abstract

Purpose. The aim of this study was to investigate the effect of linear polarized light radiation (LPLR) on mitochondrial oxidative phosphorylation impaired by hemorrhagic shock or Escherichia coli lipopolysaccharide (LPS) in skeletal muscle.

Methods. We studied the effect of LPLR on mitochondrial function of skeletal muscle by using a model of mitochondria impaired by hemorrhage or LPS. The oxygen uptake in states 3 and 4, the respiratory control ratio (RCR), and the adenosine diphosphate-to-oxygen ratio (ADP/O) were measured with a Clark oxygen electrode.

Results. Oxygen uptake in states 3 and 4, RCR, and ADP/O were significantly decreased by hemorrhage for 4 h and by LPS treatment for 12 h. Oxygen uptake in states 3 and 4 impaired by hemorrhage increased significantly from 40.1 ± 3.2 to 60.1 ± 5.4 nmol O₂·min⁻¹·mg protein⁻¹ after LPLR, and oxygen uptake in state 4 decreased significantly from 22.8 ± 2.4 to 17.7 ± 1.5 nmol O₂·min⁻¹·mg protein⁻¹ after LPLR. RCR and ADP/O were also significantly increased from 1.8 ± 0.3 and 0.9 ± 0.2 to 3.4 ± 0.3 and 1.5 ± 0.1, respectively, by LPLR. Oxygen uptake in states 3 and 4 impaired by LPS was improved from 46.6 ± 5.1 and 21.0 ± 1.9 to 53.8 ± 6.2 and 17.9 ± 2.3 nmol O₂·min⁻¹·mg protein⁻¹, respectively following LPLR. RCR and ADP/O were also elevated significantly from 2.2 ± 0.2 and 0.9 ± 0.2 to 3.0 ± 0.3 and 1.4 ± 0.2, respectively, after LPLR.

Conclusion. LPLR improved mitochondrial oxidative phosphorylation of skeletal muscle impaired by hemorrhagic shock or E. coli LPS.