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An ultrastructural study of the influence of radiation against cell line-derived human oral malignant melanoma

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Abstract

The response to radiation of the SSM-1 cell line, derived from oral malignant melanoma, was investigated by survival curve and by light and electron microscopic abservation. Survival curve of the cells was investigated by the colony formation assay. X-irradiation with 2, 4, and 8 Gy was performed against the cell line. Morphological changes of the cells at 6, 24, 72, and 120h after irradiation were examined by both light and electron microscopy. The survival curve of the SSM-1 cells showed higher radiosensitivity than that of cutaneous melanoma cell lines. Giant cells and multinucleated cells were found only 120h after 8Gy irradiation. Ultrastructural observation revealed changes in nucleus and melanosomes; melanosomes increased in number at 120h after 8Gy irradiation. Further alterations after irradiation were noticed primarily in the nucleus. Radiosensitivity of the SSM-1 cell line derived from oral malignant melanoma was higher than that of cutaneous melanoma cell lines. The results of this study may support the concept that radiotherapy is effective for oral malignant melanoma.

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Authors and Affiliations

  1. The First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, 113, Tokyo, Japan

    Masafumi Mimura, Yutaka Kimijima & Terno Amagasa

  2. Laboratory of Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan

    Yoshinobu Eishi

  3. Central Electron Microscopic Laboratory, Tokyo Medical and Dental University, Tokyo, Japan

    Shizuko Ichinose

  4. Department of Oral Surgery, Sapporo Medical University School of Medicine, Minami 1, Nishi 16, Chuo-ku, 060, Sapporo, Japan

    Nobuyuki Tanaka

  5. Second Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, 82 Inaoka-cho, 238, Yokosuka, Japan

    Yukihiko Kinoshita & Nariaki Mizutani

Authors
  1. Masafumi Mimura
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  2. Nobuyuki Tanaka
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  3. Yoshinobu Eishi
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  4. Yutaka Kimijima
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  5. Terno Amagasa
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  6. Shizuko Ichinose
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  7. Yukihiko Kinoshita
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  8. Nariaki Mizutani
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Mimura, M., Tanaka, N., Eishi, Y. et al. An ultrastructural study of the influence of radiation against cell line-derived human oral malignant melanoma. Med Electron Microsc 30, 81–87 (1997). https://doi.org/10.1007/BF01545086

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  • DOI: https://doi.org/10.1007/BF01545086

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