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Growth inhibition of suspension cultured plant cells by ribonucleases

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Abstract

Extracellular, stylar RNases (S-RNases) are produced by self-incompatible, solanaceous plants, such asNicotiana alata, and are thought to be involved in selfpollen rejection by acting selectively as toxins to selfpollen. In this study, the toxicity of RNases to other plant cells was tested by culturing cells ofN. alata andN. plumbaginifolia in the presence ofS-RNases fromN. alata. The growth of cultured cells ofN. plumbaginifolia was inhibited by theS-RNases, but viability was not affected. Growth of cultured cells of oneN. alata selfincompatibility genotype was inhibited by twoS-RNases, indicating that inhibition was not allele specific. Comparisons with the effects of inactivated RNase and other proteins, suggest that the inhibition of growth byS 2-RNase was partly, but not wholly, due to RNase activity. Heat-denaturedS 2-RNase was a very effective inhibitor of cell growth, but this inhibitory activity may be a cell surface phenomenon.

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Abbreviations

BSA:

bovine serum albumin

D:

dalton

DEPC:

diethylpyrocarbonate

FDA:

fluorescein diacetate

FPLC:

fast high-performance liquid chromatography

MES:

2-[N-morpholino] ethanesulfonic acid

RNase:

ribonuclease

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  1. Plant Cell Biology Research Centre, Botany School, University of Melbourne, 3052, Parkville, Victoria, Australia

    W. Mary Lush & Adrienne E. Clarke

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  1. W. Mary Lush
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  2. Adrienne E. Clarke
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Lush, W.M., Clarke, A.E. Growth inhibition of suspension cultured plant cells by ribonucleases. J. Plant Res. 108, 305–312 (1995). https://doi.org/10.1007/BF02344356

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  • DOI: https://doi.org/10.1007/BF02344356

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