Summary
A catalytic determination of iodine using the As(III)/Ce(IV) reaction has been developed. The difference in reaction rate due to catalysis by iodide was measured spectrophotometrically at 310 nm. Optimum reaction conditions were investigated in order to obtain a greater sensitivity in the determination of iodine. The method can be satisfactorily used for samples containing iodine in the 0.4 to 10.0 ng range, with an overall error less than 6 %. The method has been used for the determination of iodine in serum, and gives results which compare favorably with a present clinical chemistry procedure.
Zusammenfassung
Eine katalytische Jodbestimmungsmethode auf der Grundlage der As(III)/Ce(IV)-Reaktion wurde ausgearbeitet. Die durch Jodkatalyse bedingte Differenz des Reaktionsausmaßes wurde spektralphotometrisch bei 310 nm gemessen. Das Optimum der Reaktionsbedingungen für eine größtmögliche Empfindlichkeit der Jodbestimmung wurde ermittelt. Das Verfahren kann für Jodmengen zwischen 0,4 und 10,0 ng mit Erfolg verwendet werden; der durchschnittliche Fehler beträgt weniger als 6%. Jodbestimmungen im Serum nach dieser Methode stimmen mit klinisch-chemisch ermittelten Werten gut überein.
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Ke, P.J., Thibert, R.J., Walton, R.J. et al. Catalytic determination of iodine in serum at nanogram levels using the As(III)/Ce(IV) reaction. Mikrochim Acta 61, 569–581 (1973). https://doi.org/10.1007/BF01218002
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DOI: https://doi.org/10.1007/BF01218002