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Cyclic GMP generation mediated by 5-HT-2 receptors via nitric oxide-dependent pathway and its effect on the desensitization of 5-HT-2 receptors in C6 glioma cells

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Summary

Serotonin (5-HT)-2 receptor-mediated cGMP generation was investigated in comparison with calcium (Ca2+) mobilization in C6 glioma cells. 5-HT enhanced cGMP generation, and risperidone and ketanserin potently blocked the response. These results indicate that 5-HT-2 receptors are responsible for the cGMP generation. 5-HT-induced cGMP production was completely abolished by BAPTA, an intracellular Ca2+ chelating agent, or Ng-monomethyl-L-arginine (NMMA), a nitric oxide synthase (NOS) inhibitor, suggesting that 5-HT-induced cGMP generation was through nitric oxide (NO)-dependent pathway. 5-HT (10 μM)-elicited Ca2+ mobilization and cGMP generation were reduced to 40 and 15 % after pretreatment with 10 μM 5-HT for 4 hours. NMMA did not modify 5-HT-induced desensitization of either Ca2+ mobilization or cGMP generation, suggesting that NO pathway is independent of the desensitization. The present study has demonstrated the nature of 5-HT-2 receptormediated cGMP generation in C6 glioma cells.

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  1. A. Kagaya

    Present address: Department of Psychiatry and Neurosciences, Hiroshima University School of Medicine, Hiroshima, Japan

Authors and Affiliations

  1. Department of Psychiatry and Neurosciences, Hiroshima University School of Medicine, Hiroshima, Japan

    N. Motohashi, A. Kugaya, T. Yamaji, T. Hayashi, Y. Okamoto, H. Shinno, M. Takebayashi, Y. Uchitomi & S. Yamawaki

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  1. A. Kagaya
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  2. N. Motohashi
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  3. A. Kugaya
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Kagaya, A., Motohashi, N., Kugaya, A. et al. Cyclic GMP generation mediated by 5-HT-2 receptors via nitric oxide-dependent pathway and its effect on the desensitization of 5-HT-2 receptors in C6 glioma cells. J. Neural Transmission 100, 27–38 (1995). https://doi.org/10.1007/BF01276863

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  • DOI: https://doi.org/10.1007/BF01276863

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