Summary
Quantitative electron microscopic studies of the sympathetic nerve fibers of the mouse pineal demonstrated a marked diurnal rhythm in the number of the large granulated vesicles of 80 to 150 nm diameter. The number of these vesicles showed its minimum at the middle and the end of the dark period and its maximum at the end of the light period, respectively. The large non-granulated spherical and elliptical vesicles were almost constant in number throughout the day.
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Bloom, F. E. The fine structural localization of biogenic monoamines in nervous tissue. Int. Rev. Neurobiol.13, 27–66 (1970).
Bondareff, W. Submicroscopic morphology of granular vesicles in sympathetic nerves of rat pineal body. Z. Zellforsch.67, 211–218 (1965).
Duffy, P. E., Markesbery, W. R. Granulated vesicles in sympathetic nerve endings in the pineal gland: Observations on the effects of pharmacologic agents by electron microscopy. Am. J. Anat.128, 97–116 (1970).
Farrell, K. E. Fine structure of nerve fibers in smooth muscle of the vas deferens in normal and reserpinized rats. Nature217, 279–281 (1968).
Geffen, L. B., Livett, B. G. Synaptic vesicles in sympathetic neurons. Physiol. Rev.51, 98–157 (1971).
Hökfelt, T. Distribution of noradrenaline storing particles in peripheral adrenergic neurons as revealed by electron microscopy. Acta physiol. scand.76, 427–440 (1969).
Jaim-Etcheverry, G., Zieher, L. M. Cytochemistry of 5-hydroxytryptamine at the electron microscope level. II. Localization in the autonomic nerves of rat pineal gland. Z. Zellforsch.86, 393–400 (1968).
Jaim-Etcheverry, G., Zieher, L. M. Ultrastructural cytochemistry and pharmacology of 5-hydroxytryptamine in adrenergic nerve endings. I. Localization of exogenous 5-hydroxytryptamine in the autonomic nerves of the rat vas deferens. J. Pharmacol. exp. Ther.166, 264–271 (1969 a).
Jaim-Etcheverry, G., Zieher, L. M. Selective demonstration of a type of synaptic vesicle by phosphotungstic acid staining. J. Cell Biol.42, 855–860 (1969 b).
Kapeller, K., Mayor, D. The accumulation of noradrenaline in constricted sympathetic nerves as studied by fluorescence and electron microscopy. Proc. roy. Soc. B167, 282–292 (1967).
Kappers, J. A. Survey of the innervation of the epiphysis cerebri and the accessory pineal organs of the vertebrates. Prog. Brain Res.10, 87–153 (1965).
Machado, A. B. M. Straight OsO4 versus glutaraldehyde-OsO4 in sequence as fixatives for the granular vesicles in sympathetic axons of the rat pineal body. Stain Tech.42, 293–300 (1967).
Machado, A. B. M. Electron microscopy of developing sympathetic fibers in the rat pineal body. The formation of granular vesicles. Prog. Brain Res.34, 171–185 (1971).
Matsushima, S., Ito, T. Diurnal changes in sympathetic nerve endings in the mouse pineal: Semiquantitative electron microscopic observations. J. Neural Transm.33, 275–288 (1972).
Matsushima, S., Reiter, R. J. Fine structural features of adrenergic nerve fibers and endings in the pineal gland of the rat, ground squirrel and chinchilla. Am. J. Anat.148, 463–478 (1977).
Matsushima, S., Kachi, T., Mukai, S., Morisawa, Y. Functional relationships between sympathetic nerves and pinealocytes in the mouse pineal: Quantitative electron microscopic observations. Arch. histol. jap. (Suppl.)40, 279–291 (1977).
Pellegrino de Iraldi, A., Suburo, A. M. Two compartments in the granulated vesicles of the pineal nerves. In: The Pineal Gland. CIBA symposium (Wolstenholme, G. E. W., Knight, J., eds.), pp. 177–195. Edinburgh-London: Churchill Livingstone. 1971.
Spurr, A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26, 31–43 (1969).
Tranzer, J. P., Thoenen, H. Various types of amine-storing vesicles in peripheral adrenergic nerve terminals. Experientia24, 484–486 (1968).
Weibel, E. R., Kistler, G. S., Scherle, W. F. Practical stereological methods for morphometric cytology. J. Cell Biol.30, 23–38 (1966).
Wood, J. G. The effects of niamid and reserpine on the nerve endings of the pineal gland. Z. Zellforsch.145, 151–166 (1973).
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Matsushima, S., Morisawa, Y. & Mukai, S. Diurnal variation in large granulated vesicles in sympathetic nerve fibers of the mouse pineal—Quantitative electron microscopic observations. J. Neural Transmission 45, 63–73 (1979). https://doi.org/10.1007/BF01243881
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DOI: https://doi.org/10.1007/BF01243881