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In vivo labelling and axonal transport of monoamine oxidase in the rat basal ganglia using radioactive pargyline

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Summary

The enzyme monoamine oxidase was labelled in the rat striatum or substantia nigra with locally injected radioactive pargyline. The binding was prevented by a pretreatment with non-radioactive pargyline, or with a combination of clorgyline and deprenyl. Most of the MAO labelled with3H-pargyline was of the B-type, but also some MAO-A was labelled, as shown in rats pretreated with clorgyline or deprenyl separately.

Seven days after the injection of (3H)-pargyline into the striatum a significant labelling was observed in the substantia nigra. This labelling was clorgyline sensitive, indicating type A MAO, and was not present when striatal neurons were destroyed with kainic acid. Labelling of the striatum following3H-pargyline injection into the substantia nigra was also less in kainate intoxicated striata. Damage of nigral dopamine neurons with 6-hydroxydopamine did not influence the distribution of the label.

Thus by using3H-pargyline, specific labelling and axonal transport of type A MAO in striatal neurons projecting to the substantia nigra was demonstrated.

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  1. J. B. P. Gramsbergen

    Present address: Department of Biological Psychiatry, Psychiatric University Clinic, Groningen, The Netherlands

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  1. Department of Biological Psychiatry, Psychiatric University Clinic, Groningen, The Netherlands

    J. B. Sebens & J. Korf

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Gramsbergen, J.B.P., Sebens, J.B. & Korf, J. In vivo labelling and axonal transport of monoamine oxidase in the rat basal ganglia using radioactive pargyline. J. Neural Transmission 66, 21–36 (1986). https://doi.org/10.1007/BF01262955

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  • DOI: https://doi.org/10.1007/BF01262955

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