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DNA replication of parvovirus B 19 in a human erythroid leukemia cell line (JK-1) in vitro

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Summary

A major limitation of studies on the parvovirus B 19, a causative agent of transient aplastic crisis, has been the absence of appropriate cell lines permissive for the virus. In the present study, a human erythroid leukemia cell line (JK-1) was shown to support B 19 virus DNA replication in vitro. Forty-eight hours after virus inoculation of JK-1 liquid cell cultures, the average number of B 19 genome copies was estimated at 3,000 per cell by DNA dot blot analysis. The addition of erythropoietin increased B 19 copy number to 10,000 per cell. The presence of replicative forms of the B 19 virus genome was demonstrated by Southern blot analysis. Although persistent infection of B 19 virus was not observed in JK-1 cells, this culture system will be of value in elucidating the molecular basis of the erythroid specificity of parvovirus B 19.

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Author notes

  1. F. Takaku

    Present address: National Medical Center Hospital, Shinjuku-ku, Tokyo, Japan

Authors and Affiliations

  1. Department of Hematology-Oncology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo

    T. Takahashi, K. Ozawa, K. Takahashi, F. Takaku & S. Asano

  2. First Department of Internal Medicine, Gifu University School of Medicine, Gifu

    T. Takahashi & Y. Muto

  3. The Second Department of Internal Medicine, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan

    Y. Okuno & T. Takahashi

Authors
  1. T. Takahashi
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  2. K. Ozawa
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  3. K. Takahashi
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  4. Y. Okuno
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  5. T. Takahashi
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  6. Y. Muto
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  7. F. Takaku
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  8. S. Asano
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Takahashi, T., Ozawa, K., Takahashi, K. et al. DNA replication of parvovirus B 19 in a human erythroid leukemia cell line (JK-1) in vitro. Archives of Virology 131, 201–208 (1993). https://doi.org/10.1007/BF01379092

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  • DOI: https://doi.org/10.1007/BF01379092

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