Summary
After amplification by PCR, the 5′ region of the genome of hog cholera virus (HCV) strain Alfort 187 was cloned and sequenced. The nucleotide and deduced amino acid sequences were compared with the ones of other pestiviruses. By in vitro translation experiments we were able to demonstrate the protease activity of the p 20 protein of HCV.
References
Akkina R (1991) Pestivirus bovine viral diarrhea virus polypeptide: identification of new precursor proteins. Virus Res 19: 67–82
Caij A, De Smet A, Dubois N, Koenen F (1989) High titre hog cholera virus production on Cytodex microcarriers cultures. Arch Virol 105: 113–118
Collett MS, Larson R, Gold C, Strick D, Anderson D, Purchio A (1988) Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165: 191–199
Collett MS, Larson R, Belzers SK, Retzel E (1988) Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165: 200–208
Collett MS, Wiskerchen MA, Welniak E, Belzer SD (1991) Bovine viral diarrhea virus genomic organization. In: Liess B, Moennig V, Pohlenz J, Trautwein G (eds) Ruminant pestivirus infection. Arch Virol [Suppl] 3: 19–27
Darbyshire JH (1960) A serological relationship between swine fever and mucosal disease of cattle. Vet Rec 72: 331
Deng R, Brock KV (1992) Molecular cloning and nucleotide sequence of a pestivirus genome, non-cytopathic bovine viral diarrhea virus strain SD-1. Virology 191: 867–879
Dinter Z (1963) Relationship between bovine viral diarrhea virus and hog cholera virus. Zentralbl Bakteriol Parasitenk Abt 1 Orig 188: 475–486
Gubler U, Hoffmann BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680–686
Maniatis T, Fritsche EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Meyers G, Rumenapf T, Thiel HJ (1989) Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology 171: 555–567
Moormann R, Petra A, Warmerdam M, Van Der Meer B, Schaaper W, Wensvoort G, Hulst M (1990) Molecular cloning and nucleotide sequence of hog cholera virus strain brescia and mapping of the genomic region encoding envelope protein E 1. Virology 177: 184–198
Muyldermans G, San Gabriel MC, Caij A, De Smet A, Koenen F, Hamers R (1992) Polymerase chain reaction mediated cloning and expression of the structural proteins of hog cholera virus inE. coli. Second symposium on ruminant pestiviruses, Annecy 1992. European Society for Veterinary Virology, Annecy, p 78
Muyldermans G, Caij A, De Smet A, Koenen F, Hamers R (1993) Characterization of structural and non-structural proteins of hog cholera virus by means of monoclonal antibodies. Arch Virol 131: 405–417
Renard A, Dina D, Martial J (1987) Vaccines and diagnostics derived from bovine diarrhea virus. European Patent Application No. 86870095.6. Publication No. 0208672, 14 January
Ruggli N, Mitchell DM, Tratschin JD (1992) Cloning and expression of hog cholera virus structural proteins. Second symposium on ruminant pestiviruses, Annecy, 1992. European Society for Veterinary Virology, Annecy, p 82
Sambrook J, Fritch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Thiel HJ, Stark R, Weiland E, Rümenapf T, Meyers G (1991) Hog cholera virus: molecular compositions of virions from a pestivirus. J Virol 65: 4705–4712
Westaway EG, Brinton MA, Gaidamovich SYA, Horzinek MC, Igarashi A, Kaäriainen L, Lvov DK, Porterfield JS, Russell PK, Trent DW (1985) Flaviviridae. Intervirology 24: 125–139
Wiskerchen EG, Belzer S, Collett MS (1991) Pestivirus gene expression: the first protein product of the bovine viral diarrhea virus large open reading frame, p 20 possesses proteolytic activity. J Virol 65: 4508–4514
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Muyldermans, G., San Gabriel, M.C., Caij, A. et al. Polymerase chain reaction-mediated cloning and in vitro translation of the genes coding for the structural proteins of hog cholera virus. Archives of Virology 132, 429–435 (1993). https://doi.org/10.1007/BF01309551
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309551