Summary
To characterize the role of the humoral immune response on HIV-1 infection of monocytes and macrophages (Mφs) we examined the susceptibility of in vitro cultured monocyte/Mφs to various HIV-1 isolates and the influence of heterologous and particularly autologous anti HIV-1 sera on this infection. Depending on the period of in vitro cultivation and the virus isolate used different patterns of susceptibility were detected. One week old monocyte/Mφs were highly susceptible to HIV-1 infection, in contrast to monocyte/Mφs cultured 4 weeks. The infection by virus isolated immediately after seroconversion lead to persistent infection with high level of antigen production in contrast to infection by homologous virus isolated later. MAb against the V3-IIIB loop and sCD4 inhibited the infection of monocyte/Mφs in a dose dependent manner, indicating that infection requires binding to CD4 and that post binding events may be common to the infection of lymphocytes. Anti HIV-1 sera showed neutralizing activity against heterologous and even autologous escape virus. This finding, together with the observation that monocytes and Mφs are infected in vivo, suggests that protection against HIV-1 infection of monocytes and Mφs in vivo may not be obtainable by the humoral immune response alone.
Similar content being viewed by others
References
Albert J, Abrahamsson B, Nagy K, Aurelius E, Gaines H, Nyström G, Fenyö EM (1990) Rapid development of isolate-specific neutralizing antibodies after primary HIV-1 infection and consequent emergence of virus variants which resist neutralization by autologous sera. AIDS 4: 107–112
Arendrup M, Ulrich K, Nielsen JO, Lindhardt BØ, Kusk P, Mathiesen LR, Pedersen C, Krogsgaard K (1989) HIV neutralizing antibodies: development and association with HIV related disease. Scand J Infect Dis 21: 19–26
Arendrup M, Nielsen CM, Hansen J-ES, Mathiesen L, Lindhardt BØ, Scheibel E, Nielsen JO (1992) Neutralizing antibodies against two HIV-1 strains in consecutively collected serum samples: cross neutralization and association to HIV-1 related disease. Scand J Infect Dis 24: 21–28
Arendrup M, Nielsen C, Hansen J-ES, Pedersen C, Mathiesen L, Nielsen JO (1992) Autologous HIV-1 neutralizing antibodies: Emergence of neutralization-resistant escape virus and subsequent development of escape virus neutralizing antibodies. J Acquir Immune Defic Syndr 5: 303–307
Arendrup M, Sönnerborg A, Svennerholm B, Åkerblom L, Nielsen C, Clausen H, Olofsson S, Nielsen JO, Hansen J-ES (1993) Neutralizing antibody response during human immunodeficiency virus type 1 infection: type and group specificity and viral escape. J Gen Virol 74: 855–863
Collman R, Hassan NF, Walker R, Godfrey B, Cutilli J, Hastings JC, Friedman H, Douglas SD, Nathanson N (1989) Infection of monocyte-derived macrophages with human immunodeficiency virus type 1 (HIV-1). J Exp Med 170: 1149–1163
de Jong, J-J, Goudsmit J, Keulen W, Klaver B, Krone W, Tersmette M, de Ronde A (1992) Human immunodeficiency virus type 1 clones chimeric for the envelope V3 domain differ in syncytium formation and replication capacity. J Virol 66: 757–765
Fouchier RAM, Groenink M, Kootstra NA, Tersmette M, Huisman HG, Miedema F, Schuitemaker H (1992) Phenotype-associated sequence variation in the third variable domain of the human immunodeficiency virus type 1 gp120 molecule. J Virol 66: 3183–3187
Gartner S, Markovits P, Markovits DM, Kaplan MH, Gallo RC, Popovic M (1986) The role of mononuclear phagocytes in HTLV-III/LAV infection. Science 233: 215–219
Gendelman HE, Baca LM, Husayni H, Turpin JA, Skillman D, Kalter DC, Orenstein JM, Hoover DL, Meltzer MS (1990) Macrophage-HIV interaction: viral isolation and target cell tropism. AIDS 4: 221–228
Ho W-Z, Lioy J, Song L, Cutilli JR, Polin RA, Douglas SD (1992) Infection of cord blood monocyte-derived macrophages with human immunodeficiency virus type 1. J Virol 66: 573–579
Joualt T, Chapuis F, Olivier R, Parravicini C, Bahraoui E, Gluckman J-C (1989) HIV infection of monocytic cells: role of the antibody-mediated virus binding to Fc-gamma receptors. AIDS 3: 125–133
Kazazi F, Mathijs JM, Foley P, Cunningham AL (1989) Variations in CD4 expression by human monocytes and macrophages and their relationship to infection with the human immunodeficiency virus. J Gen Virol 70: 2661–2672
Klatzmann D, Barré-Sinnoussi F, Nugeyre MT, Dauget C, Vilmer E, Griscelli C, Brun-Vezinet F, Rouzioux C, Gluckman JC, Cheman J-C, Montagnier L (1984) Selective tropism of lymphadenopathy associated virus (LAV) for helper-induvcer T lymphocytes. Science 225: 59–63
Larkin M, Childs RA, Matthews TJ, Thield S, Mizuochi T, Lawson AM, Savill JS, Haslett C, Diaz R, Feizi T (1989) Oligosaccharide-mediated interactions of the envelope glycoprotein gp120 of HIV-1 that are independent of CD4 recognition. AIDS 3: 793–798
Linsley PS, Ledbetter JA, Kinney-Thomas E, Hu SL (1988) Effects of anti-gp 120 monoclonal antibodies on CD4 receptor binding by the env protein of human immunodeficiency virus type 1. J Virol 62: 3695–3702
Löwer J, Davidson EA, Teich NM, Weiss RA, Joseph AP, Kurth R (1981) Heterophil human antibodies recognize oncovirus envelope antigens: epidemiological parameters and immunological specificity of the reaction. Virology 109: 409–417
McDougal JS, Kennedy MS, Sligh JM, Cort SP, Mawle A, Nicholson JKA (1986) Binding of HTLV-III/LAV to T4 + T cells by a complex of the 110 K viral protein and the T4 molecule. Science 231: 382–385
McElrath MJ, Pruett JE, Cohn ZA (1989) Mononuclear phagocytes of blood and bone marrow: Comparative roles as viral reserviors in human immunodeficiency virus type 1 infections. Proc Natl Acad Sci USA 86: 675–679
Nara PL, Smit L, Dunlop N, Hatch W, Merges M, Waters D, Kelliher J, Gallo RC, Fischinger PJ, Goudsmit J (1990) Emergence of viruses resistant to neutralization by V3-specific antibodies in experimental human immunodeficiency virus type 1 IIIB infection of chimpanzees. J Virol 64: 3779–3791
Nelson GW, Perelson AS (1992) A mechanism of immune escape by slow-replication HIV strains. J Acquir Immune Defic Syndr 5: 82–93
Nielsen C, Pedersen C, Lundgren JD, Gerstoft J (1993) Biological properties of HIV-isolates in primary HIV-infection: consequences for subsequent course of infection. AIDS 7: 1035–1040
Nielsen CM, Bygbjerg IC, Vestergaard BF (1987) Detection of HIV antigens in eluates from whole blood collected on filterpaper. Lancet i: 566–567
Perno C-F, Baseler MW, Broder S, Yarchoan R (1990) Infection of monocytes by human immunodeficiency virus type 1 blocked by inhibitors of CD4-gp 120 binding, even in the presence of enhancing antibodies. J Exp Med 171: 1043–1056
Shadduck PP, Weinberg B, Haney AF, Bartlett JA, Langlois AJ, Bolognesi DP, Matthews TJ (1991) Lack of enhancing effect of human anti-human immunodeficiency virus type 1 (HIV-1) antibody on HIV-1 infection of human blood monocytes and peritoneal macrophages. J Virol 65: 4309–4316
Takeda A, Sweet RW, Ennis FA (1990) Two receptors are required for antibody-dependent enhancement of human immunodeficiency virus type 1 infection: CD4 and Fc gamma R. J Virol 64: 5605–5610
Tremblay M, Wainberg MA (1990) Neutralization of multiple HIV-1 isolates from a single subject by autologous sequential sera. J Infect Dis 162: 735–737
Valentin A, Albert J, Fenyö EM, Åsjø B (1990) HIV-1 infection of normal human macrophage cultures: implication for silent infection. Virology 177: 790–794
Valentin A, Von Gegerfelt A, Matsuda S, Nilsson K, Åsjö B (1991) In vitro maturation of mononuclear phagocytes and susceptibility to HIV-1 infection. J Acquir Immune Defic Syndr 4: 751–759
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Arendrup, M., Olofsson, S., Nielsen, J.O. et al. HIV-1 infection of in vitro cultured human monocytes: early events and influence of anti HIV-1 antibodies. Archives of Virology 136, 35–52 (1994). https://doi.org/10.1007/BF01538815
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01538815