Skip to main content
SpringerLink
Log in

Induction of cellular DNA synthesis during lytic infection with SV 40: A function of viral genome

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

Induction of cellular DNA replication during the lytic infection of AGMK cells with SV 40 can be prevented, by interferon. UV-light irradiated SV 40 or viral coat protein has no DNA stimulating activity.

These characteristics suggest the stimulation of cellular DNA synthesis to be mediated by the expression of the viral genome, and not by a constituent of the infecting virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AGMK:

African Green Monkey kidney

DEAEdextran:

diethylaminoethyl dextran

FUdR:

S-fluoro-2′-deoxy-uridine

IUdR:

5-iodo-2′-deoxy-uridine

PBS:

phosphate buffered salt solution

Poly I∶C:

polyinosinic: polycytidylic acid

VSV:

vesicular stomatitis virus

References

  1. Boehlandt, D., G. Brandner, andJ. Burger: Inhibition of DNA and RNA synthesis in SV 40 infected African Green Monkey kidney cell cultures by interferon. Prpc. 5th Int. Congr. Infect. Dis. (Vienna August-Sept. 1970)1, 59 (1970).

    Google Scholar 

  2. Brandner, G., and J.Burger: unpublished results (1970).

  3. Büttner, W.: unpublished results (1969).

  4. Cantell, K.: Preparation of human leukocyte Interferon. Symposia Series in Immunobiological Standardization14, 6 (1970).

    Google Scholar 

  5. Colter, J. S., R. A. Brown, andK. A. O. Ellem: Observation on the use of phenol for the isolation of deoxyribunucleicacid. Biochem. biophys. Acta (Amst.)55, 31 (1962).

    Google Scholar 

  6. Dianzani, F., G. Rita, D. Cantagalli, andS. Gagnoni: Effect of DEAE-dextran on interferon production and protective effect in mice treated with the double-stranded polynucleotide complex polyinosinic-polycytidylic acid. J. Immunol.102, 24 (1969).

    Google Scholar 

  7. Dulbecco, R., L. H. Hartwell, andM. Vogt: Induction of cellular DNA synthesis by polyoma virus. Proc. nat. Acad. Sci. (Wash.)53, 403 (1965).

    Google Scholar 

  8. Dulbecco, E., andT. Johnson: Interferon-sensitivity of the enhanced incorporation of thymidine into cellular DNA induced by polyoma virus. Virology42, 368 (1970).

    Google Scholar 

  9. Hatanaka, M., andR. Dulbecco: Induction of DNA synthesis by SV 40. Proc. nat. Acad. Sci. (Wash.)56, 736 (1966).

    Google Scholar 

  10. Kit, S., R. A. De Torres, D. R. Dubbs, andM. L. Salvi: Induction of cellular deoxyribonucleic acid synthesis by simian virus 40. J. Virol.1, 738 (1967).

    Google Scholar 

  11. Levy, H. B., andW. A. Carter: Molecular basis of the action of interferon. J. molec. Biol.31, 561 (1968).

    Google Scholar 

  12. McChutchan, J. H., andJ. S. Pagano: Enhancement of the infectivity of simian virus 40 deoxyribunucleic acid with diethylaminoethyl-dextran. J. nat. Cancer Inst.41, 351 (1968).

    Google Scholar 

  13. Ritzi, E., andA. J. Levine: Deoxyribunucleic acid replication in Simian virus 40-infected cells. III. Comparison of Simian Virus 40 lytic infection in three different monkey kidney cell lines. J. Virol.5, 686 (1970).

    Google Scholar 

  14. Todaro, G. J., andH. Green: Interferon resistance of SV 40 induced DNA synthesis. Virol.33, 752 (1967).

    Google Scholar 

  15. Werchau, H., H. Westphal, G. Maass undR. Haas: Untersuchungen über den Nucleinsäurestoffwechsel von Affennierengewebekulturen nach Infektion mit SV 40. II. Einfluß der Infektion auf den DNS-Stoffwechsel. Arch. ges. Virusforsch.19, 351 (1966).

    Google Scholar 

  16. Westphal, H., H. Werchau, G. Maass undR. Haas: Untersuchungen über den Nucleinsäurestoffwechsel von Affennierengewebe-Kulturzellen nach Infektion mit SV 40. I. Darstellung und Chromatographie der SV 40-DNS. Arch. ges. Virusforsch.19, 342 (1966).

    Google Scholar 

Download references

Author information

Author notes

  1. G. Brandner

    Present address: Sonderforschungsbereich Medizinische Virologie der Deutsehen Forschungsgemeinschaft, Freiburg Breisgau, BRD

Authors and Affiliations

  1. Sonderforschungsbereich Medizinische Virologie der Deutsehen Forschungsgemeinschaft, Freiburg Breisgau, BRD

    D. Boehlandt, J. Burger & M. Leveringhaus

Authors
  1. G. Brandner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Boehlandt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Burger
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Leveringhaus
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The interferon part of this paper was presented in short form at the 5th Int. Congr. Infect. Dis., Vienna, August 1970. (Boehlandtet al., 1970).

The experiments are parts of the thesis of D.B., J.B., and M.L.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brandner, G., Boehlandt, D., Burger, J. et al. Induction of cellular DNA synthesis during lytic infection with SV 40: A function of viral genome. Archiv f Virusforschung 34, 323–331 (1971). https://doi.org/10.1007/BF01242978

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01242978

Keywords

Search

Navigation