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Biological activity of 2-phenylethanol and its derivatives

VIII. Influence on herpesvirus DNA-synthesis in rabbit kidney cells

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Summary

The biosynthesis of herpesvirus DNA in rabbit kidney cells is inhibited to 50% by PEA (2-Phenylethanol) at 0.65 mg PEA/ml. The inhibition of cellular DNA synthesis in uninfected cells by PEA is about twice as sensitive as that of viral DNA synthesis.

The cellular DNA-dependent DNA polymerase is inhibited in a non-competitive way. The 50% inhibitory concentration amounts to 0.8 mg PEA/ml.

In contrast the herpesvirus induced DNA-dependent DNA polymerase is 10-fold more resistant towards PEA.

It is assumed that, contrary to the synthesis of cellular DNA, the PEA induced inhibition of viral DNA synthesis is not caused by an inhibition of the virus-induced DNA-dependent DNA polymerase.

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References

  1. Berrah, G., andW. A. Konetzka: Selective and reversible inhibition of the synthesis of bacterial deoxyribonucleic acid by phenethyl alcohol. J. Bact.83, 738 (1962).

    PubMed  Google Scholar 

  2. Bollum, F. J.: Filter paper disc techniques for assay in radioactive macromolecules. In: Procedures in Nucleic Acid Research (G. J. Cantoni andD. R. Davis, eds.) p. 296. New York-London: Harper and Row, 1966.

    Google Scholar 

  3. Bowen, J. M., R. G. Hughes, andL. Dmochowski: Studies on the inhibition of polyoma virus replicationin vitro: Comparison of the effects of phenethyl alcohol, 5-iodo-2-deoxyuridine, puromycin and actinomycin D. Tex. Tep. Biol. Med.24, 143 (1966).

    Google Scholar 

  4. Burns, V. W.: Effects of phenethyl alcohol on yeast cells. J. cell. Physiol.72, 97 (1968).

    PubMed  Google Scholar 

  5. Dixon, M., andE. C. Webb: Enzymes. London: Longmans, 1966.

    Google Scholar 

  6. Falke, D.: Ca++, Histidin und Zn++ als Faktoren bei der Riesenzellbildung durch Herpesvirus hominis. Z. Med. Mikrobiol. Immunol.153, 175 (1967).

    PubMed  Google Scholar 

  7. Gornall, A. G., G. J. Bardawill, andM. M. David: Determination of serum proteins by means of the biuret reaction. J. biol. Ohem.177, 751 (1949).

    Google Scholar 

  8. Higgins, M. L., T. J. Shaw, M. C. Tillman, andF. R. Leach: Effect of phenethyl alcohol on cell culture, growth. II. Isolated cell components and lysosomal enzymes. Exp. Cell Res.56, 24 (1969).

    PubMed  Google Scholar 

  9. Keir, H. M.: DNA Polymerases from mammalian cells. In: Progress in Nucleic Acid Research and Molecular Biology (J. N. Davidson andW. E. Cohn, eds.)4, 81. New York: Academic Press, 1965.

    Google Scholar 

  10. Keir, H. M., J. M. Morrison, andH. Subak-Sharpe: Altered properties of deoxyribonucleic acid nucleotidyltransferase after infection of mammalian cells with herpes simplex virus. Nature (Lond.)210, 369 (1966).

    Google Scholar 

  11. Keir, H. M.: Virus-induced enzymes in mammalian cells infected with DNA-viruses. In: The Molecular Biology of Viruses; p. 67. XVIII Symp. gen. Microbiol. University Press, Cambridge, 1968.

    Google Scholar 

  12. Kirby, K. S., andE. A. Cook: Isolation of deoxyribonucleic acid from mammalian tissues. Biochemical J.104, 254 (1967).

    Google Scholar 

  13. Kissane, J. M., andE. Robins: The fluorometric measurement of deoxyribonucleic acid in animal tissue with special reference to the central nervous system. J. biol. Chem.233, 184 (1958).

    PubMed  Google Scholar 

  14. Koller, S.: In: Biochemisches Taschenbuch (H. M. Rauen, ed.). Berlin, Göttingen, Heidelberg: Springer Verlag, 1956.

    Google Scholar 

  15. Konetzka, W. A., andG. Berrah: Inhibition of replication of bacteriophage T2 by phenethylalcohol. Biochem. biophys. Res. Commun.8, 407 (1962).

    Google Scholar 

  16. Leach, F. R., N. H. Best, E. M. Davis, D. C. Sanders, andD. M. Gimlin: Effect of phenethyl alcohol on cell culture growth. I. Characterization of the effect. Exp. Cell Res.36, 524 (1964).

    PubMed  Google Scholar 

  17. Lester, G.: Inhibition of growth, synthesis and permeability in neurospora crassa by phenethyl alcohol. J. Bact.90, 29 (1965).

    Google Scholar 

  18. Lilly, B. O., andJ. H. Brewer: The selective antibacterial action of phenethyl alcohol. J. Amer. pharm. Ass. sci. Ed.42, 6 (1953).

    Google Scholar 

  19. Maass, G., andJ. Hempel: Reversible Hemmung der Vermehrung des Virus SV-40 durch aromatische Alkohole. Z. med. Mikrobiol. Immunol.152, 45 (1966).

    PubMed  Google Scholar 

  20. Morrison, J. M.: Studies on mammalian viral DNA and its metabolism with special reference to herpes virus. Ph.D. Thesis, University of Glasgow, 1967.

  21. Müller, W. E. G., B. Heicke, andR. K. Zahn: Biological activity of β-phenylethanol and its derivatives. V. Influence on DNA and RNA synthesis in different in vitro systems. Biochim. biophys. Acta (Amst.)240, 506 (1971).

    Google Scholar 

  22. Nitsan, J., andA. Lang: Inhibition of cell division and cell elongation in higher plants by inhibition of DNA synthesis. Develop. Biol.12, 358 (1965).

    PubMed  Google Scholar 

  23. Nonoyama, M., andY. Ikeda: Inhibition of RNA phage growth by phenethyl alcohol. Biochem. biophys. Res. Commun.15, 87 (1964).

    PubMed  Google Scholar 

  24. Plagemann, P. G. W.: Reversible inhibition of synthesis of macromolecules and disaggregation of polysomes in rat hepatoma cells. Biochim. biophys. Acta (Amst.)155, 202 (1968).

    Google Scholar 

  25. Plagemann, P. G. W.: Reversible inhibition of mengovirus RNA polymerase and of virus maturation in Novikoff rat hepatoma cells by phenethyl alcohol. Virology34, 319 (1968).

    PubMed  Google Scholar 

  26. Plagemann, P. G. W.: Phenethyl alcohol: reversible inhibition of synthesis of macromolecules and disaggregation of polysomes in rat hepatoma cells. Biochim. biophys. Acta (Amst.)155, 202 (1968).

    Google Scholar 

  27. Roizman, B.: Reversible inhibition of herpes simplex multiplication in HEp-2 cells with phenethyl alcohol. Virology19, 580 (1963).

    PubMed  Google Scholar 

  28. Zahn, R. K., E. Tiesler, A. K. Kleinschmidt, andD. Lang: Ein Konservierungs- und Darstellungs-Verfahren für Desoxyribonucleinsäuren und ihre Ausgangsmaterialien. Biochem. Z.336, 281 (1962).

    PubMed  Google Scholar 

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Author notes

  1. B. Heicke

    Present address: Institut für Physiologische Chemie, Johannes-Gutenberg-Universität, Mainz, Federal Republic of Germany

Authors and Affiliations

  1. Institut für Physiologische Chemie, Johannes-Gutenberg-Universität, Mainz, Federal Republic of Germany

    W. E. G. Müller, D. Falke & R. K. Zahn

  2. Institut für Medizinische Mikrobiologie, Johannes-Gutenberg-Universität, Mainz, Federal Republic of Germany

    W. E. G. Müller, D. Falke, B. Heicke & R. K. Zahn

Authors
  1. W. E. G. Müller
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  2. D. Falke
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  3. B. Heicke
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  4. R. K. Zahn
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With technical assistance by R.Beyer

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Müller, W.E.G., Falke, D., Heicke, B. et al. Biological activity of 2-phenylethanol and its derivatives. Archiv f Virusforschung 40, 205–214 (1973). https://doi.org/10.1007/BF01242539

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  • DOI: https://doi.org/10.1007/BF01242539

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