Summary
The sequence of human CMV infection in WI 38 cells at high input multiplicity was studied by electron microscopy.
The majority of entering virus particles were degraded within the cytoplasmic vacuoles but few particles released their capsids into the cytoplasm. Unenveloped particles with a distinct core were observed to persist over a period of 24 hours post-infection. Virus particles in the nucleus were only detectable 48 hours post-infection and thereafter. Process of formation and segregation of primary lysosome-like dense bodies was observed in association with the termination of the virus assembly cycle.
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References
Brandes, D.: Observation on the apparent mode of formation of pure lysosomes. J. Ultrastruct. Res.12, 63–80 (1965).
Cohn, Z. A., andM. E. Fedorko: The formation and fate of lysosomes. In: “Lysosomes in Biology and Pathology” (J. T. Dingle andH. B. Fell, eds.), Vol. 1, 43–63. New York: American Elsevier Publishing Co., Inc., 1969.
Dales, S.: Penetration of animal viruses into cells. In: “Progress in Medical Virology” (J. L. Melnick, ed.), Vol. 7, 1–43. New York: S. Karger, 1965.
Furukawa, T., andS. Plotkin: Some characteristics of high titer CMV from infected WI38. Fed. Proc. (Abst)31, 3352 (1972).
Halsted, C. C., A. B. Minnefor, andP. S. Lietman: Inhibition of cytomegalovirus by Rifampin. J. infect. Dis.125, 552–555 (1972).
Luse, S. A., andM. G. Smith: Electron microscopy of salivary gland viruses. J. exp. Med.107, 623–632 (1958).
Luse, S. A., andM. G. Smith: Electron microscope studies of cells infected with the salivary gland viruses. Ann. N.Y. Acad. Sci.81, 133–144 (1959).
McAllister, R. M., R. M. Straw, J. E. Filbert, andC. R. Goodheart: Human cytomegalovirus. Cytochemical observations of intracellular lesion development correlated with viral synthesis and release. Virology19, 521–531 (1963).
McGavran, M. H., andM. G. Smith: Ultrastructural cytochemical and microchemical observations on cytomegalovirus (salivary gland virus) infection of human cells in tissue culture. Exp. molec. Path.4, 1–10 (1965).
Miyamoto, K., andC. Morgan: Structure and development of viruses as observed in the electron microscope. XI. Entry and uncoating of Herpes simplex virus. J. Virol.8, 910–918 (1971).
Morgan, C., H. M. Rose, andB. Mednis: Electron microscopy of Herpes simplex virus. I. Entry. J. Virol.2, 507–516 (1968).
Ruebner, B. H., T. Hirano, R. J. Slusser, andD. N. Medearis, Jr.: Human cytomegalovirus infection: Electron microscopic and histochemical changes in cultures of human fibroblasts. Amer. J. Path.46, 477–496 (1965).
Ruebner, B. H., T. Hirano, R. J. Slusser, J. Osborn, andD. N. Medearis, Jr.: Cytomegalovirus infection: Viral ultrastructure with particular reference to the relationship of lysosomes to cytoplasmic inclusions. Amer. J. Path.48, 971–989 (1966).
Venable, J. H., andR. Coggeshall: A simple lead citrate stain for electron microscopy. J. Cell Biol.25, 407–408 (1965).
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This investigation was supported, in part, by grants from the John A. Hartford Foundation and Public Health Service Research Grant #NS06859 from the National Institute of Neurological Diseases and Stroke.
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Iwasaki, Y., Furukawa, T., Plotkin, S. et al. Ultrastructural study on the sequence of human cytomegalovirus infection in human diploid cells. Archiv f Virusforschung 40, 311–324 (1973). https://doi.org/10.1007/BF01242551
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DOI: https://doi.org/10.1007/BF01242551