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Virological and immunofluorescent studies on the multiplication of sindbis virus in L cells

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Summary

Sindbis virus has been adapted to L cells in the course of serial passages until titre of infectivity and degree of cytopathic effect remained constant. The growth curve shows a latent period of 1 to 2 hours followed by an exponential rise of virus multiplication. Peak titre is reached 7 to 8 hours after infection.

By means of immunofluorescence technique localization of the antigens of Sindbis virus in the infected cell was studied at various times after infection. Viral antigens are detectable exclusively in the cytoplasm. They appear as early as 1 hour after infection in a few cells, at first diffusely distributed, and beginning with the 4th hour, increasing in the form of fluorescent granules. These granules are supposed to represent accumulations of viral nucleocapsids at the surface of cytoplasmic vacuoles as well as mature virus within the vesicles as revealed by electron microscopic studies with several group A arboviruses (loc. cit.). Percentage of cells containing viral antigens reaches its maximum at 5 to 6 hours. At later stages of infection cells contain only a few big fluorescent aggregates.

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Author notes

  1. Anneliese Veckenstedt & M. Wagner

    Present address: Akademie der Wissenschaften der DDR, Forschungszentrum für Molekularbiologie und Medizin Zentralinstitut für Mikrobiologie und experimentelle Therapie Jena, German Democratic Republic

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    1. Anneliese Veckenstedt
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    2. M. Wagner
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    Veckenstedt, A., Wagner, M. Virological and immunofluorescent studies on the multiplication of sindbis virus in L cells. Archiv f Virusforschung 42, 144–153 (1973). https://doi.org/10.1007/BF01270834

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    • DOI: https://doi.org/10.1007/BF01270834

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