Summary
Comparison of methods to inactivate lytic properties of herpes simplex viruses revealed that ultraviolet irradiation, photodynamic procedures, and heat all destroyed infectivity effectively. Ability to biochemically transform thymidine kinase deficient cells to an enzyme positive phenotype was retained after limited exposure to heat or ultraviolet light but appeared to be destroyed by photodynamic methods employing neutral red. Exposure to 56°C quickly and effectively destroyed transforming activity with lower temperatures being less effective. The most reproducible transforming assays were obtained following inactivation by ultraviolet light. Cell cultures developed by this procedure were virus-free but retained ability to synthesize virus-specific antigens.
Similar content being viewed by others
References
Albrecht, T., St. Jeor, S. C., Funk, F. D., Rapp, F.: Multiplicity reactivation of human cytomegalovirus inactivated by ultraviolet light. Int. J. Radiat. Biol.26, 445–454 (1974).
Darai, G., Munk, K.: Neoplastic transformation of rat embryo cells with herpes simplex virus. Int. J. Cancer18, 469–481 (1976).
Darai, G., Braun, R., Flügel, R. M., Munk, K.: Malignant transformation of rat embryo fibroblasts by herpes simplex virus types 1 and 2 at suboptimal temperature. Nature265, 744–746 (1977).
Davidson, R. L., Adelstein, S. J., Oman, M. N.: Herpes simplex virus as a source of thymidine kinase for thymidine kinase-deficient mouse cells: suppression and reactivation of the viral enzyme. Proc. Nat. Acad. Sci.70, 1912–1916 (1973).
Duff, R., Rapp, F.: Properties of hamster embryo fibroblasts transformedin vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2. J. Virol.8, 469–477 (1971).
George, J., Devoret, R., Radman, M.: Indirect ultraviolet-reactivation of phage lambda. Proc. Nat. Acad. Sci. U.S.A.71, 144–147 (1974).
Howett, M. K., Miller, R. L., Rapp, F.: Herpesvirus transformation: inability of infectious virus to mediate transformation in the presence of inactivated virus. (Manuscript in preparation.)
Lin, S., Munyon, W.: Expression of the viral thymidine kinase gene in herpes simplex virus-transformed L cells. J. Virol.14, 1199–1208 (1974).
Littlefield, J. W.: Studies on thymidine kinase in cultured mouse fibroblasts. Biochim. biophys. Acta95, 14–22 (1965).
Lytle, C. D.: Host-cell reactivation in mammalian cells. I. Survival of ultraviolet-irradiated herpes virus in different cell lines. Int. J. Radiat. Biol.19, 329 to 337 (1971).
Macnab, J. C. M.: Transformation of rat embryo cells by temperature-sensitive mutants of herpes simplex virus. J. gen. Virol.24, 143–153 (1974).
Macpherson, I., Montagnier, L.: Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology23, 291–294 (1964).
Munyon, W., Kraiselburd, E., Davis, D., Mann, J.: Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus. J. Virol.7, 813–820 (1971).
Plummer, G., Lewis, B.: Thermoinactivation of herpes simplex virus and cytomegalovirus. J. Bacteriol.89, 671–674 (1965).
Pope, J. H., Rowe, W. P.: Detection of specific antigen in SV 40-transformed cells by immunofluorescence. J. exp. Med.120, 121–128 (1964).
Rapp, F.: Variants of herpes simplex virus: isolation, characterization, and factors influencing plaque formation. J. Bacteriol.86, 985–991 (1963).
Rapp, F., Buss, E. R.: Comparison of herpes simplex isolates using a quantitative selection assay for transformation. Intervirology6, 72–82 (1975/76).
Rapp, F., Li, J. L. H., Jerkofsky, M.: Transformation of mammalian cells by DNA-containing viruses following photodynamic inactivation. Virology55, 339–346 (1973).
Ross, L. J. N., Cameron, K. R., Wildy, P.: Ultraviolet irradiation of herpes simplex virus: reactivation processes and delay in virus multiplication. J. gen. Virol.16, 299–311 (1972).
Ross, L. J. N., Wildy, P., Cameron, K. R.: Formation of small plaques by herpes viruses irradiated with ultraviolet light. Virology45, 808–812 (1971).
Roubal, J., Vonka, V.: Multiplicity reactivation in UV-irradiated herpes simplex type 1 virus. Intervirology1, 73–79 (1973).
Wallis, C., Scheiris, C., Melnick, J. L.: Photodynamically inactivated vaccines prepared by growing viruses in cells containing neutral red. J. Immunol.99, 1134–1139 (1967).
Wallis, C., Melnick, J.: Thermostabilization and thermosensitization of herpesvirus. J. Bacteriol.90, 1632–1637 (1965).
Author information
Authors and Affiliations
Additional information
With 7 Figures
Rights and permissions
About this article
Cite this article
Rapp, F., Turner, N. Biochemical transformation of mouse cells by herpes simplex virus types 1 and 2: Comparison of different methods for inactivation of viruses. Archives of Virology 56, 77–87 (1978). https://doi.org/10.1007/BF01317284
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317284