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Quantitative analysis of defective interfering particles in infectious pancreatic necrosis virus preparations

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Summary

Infectious pancreatic necrosis virus exhibited an interference phenomenon that resulted in the survival of the infected cell with one hit kinetics. The responsible factor was found to co-purify with standard virus through a purification regime that employed two CsCl gradients and a sucrose gradient. This result suggested that a defective interfering (DI) viral particle was involved. It was possible to estimate the number of DI particles by a statistical method using the Poisson distribution that related cell survival to input DI/cell, which indicated that virus samples from dilute passage contained as many DI particles as samples from undiluted passage; this means that multiple undiluted virus passage did not increase the yield of DI particles. In isopycnic CsCl gradient centrifugation, the DI particles were found in a broad band superimposed over the standard virus peak and extending above it, such that the ratio DI/PFU varied from 0.3–20 in different fractions. These centrifugation methods did not completely separate DI particles from standard virus.

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Authors and Affiliations

  1. Department of Microbiology, University of Alberta, Edmonton, Alberta, Canada

    R. D. Macdonald & T. Yamamoto

  2. Department of Biology, University of Calgary, T2N 1N4, Calgary, Alberta, Canada

    R. D. Macdonald

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  1. R. D. Macdonald
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  2. T. Yamamoto
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Macdonald, R.D., Yamamoto, T. Quantitative analysis of defective interfering particles in infectious pancreatic necrosis virus preparations. Archives of Virology 57, 77–89 (1978). https://doi.org/10.1007/BF01315639

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  • DOI: https://doi.org/10.1007/BF01315639

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