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Molecular cloning of an endogenous cat retroviral element (ECE 1)—a recombinant between RD-114 and FeLV-related sequences

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Summary

A lambda L 47.1 clone library was constructed from BamHI digests of cellular cat DNA and was screened with cDNA probes specific for the 3'-terminus of the replication competent RD-114 virus. The first analysis of one clone (λ-ECE 1) shows that it consists at least of a 3'-LTR and a 5' adjacent env gene. The LTR nucleotide sequences of the RD-114 cDNA clones and ECE 1 are identical within the R region and unrelated to FeLV. Otherwise, the region upstream from the 3'-LTR of ECE 1 shows homology to the transmembrane protein encoding gene of FeLV, but not to RD-114. These results imply that ECE 1 is a recombinant between an RD-114-like 3'-LTR and a FeLV-related env portion.

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Authors and Affiliations

  1. Department of Cell Differentiation, Central Institute of Molecular Biology, Academy of Sciences of the German Democratic Republic, Berlin, German Democratic Republic

    W. Beyer, Regina Möhring, B. Drescher, U. Nötzel & Sinaida Rosenthal

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  1. W. Beyer
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  2. Regina Möhring
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  3. B. Drescher
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  4. U. Nötzel
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  5. Sinaida Rosenthal
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Beyer, W., Möhring, R., Drescher, B. et al. Molecular cloning of an endogenous cat retroviral element (ECE 1)—a recombinant between RD-114 and FeLV-related sequences. Archives of Virology 96, 297–301 (1987). https://doi.org/10.1007/BF01320971

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  • DOI: https://doi.org/10.1007/BF01320971

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