Summary
The effects of light intensity and cell age on the greening of etioplasts were studied in seedlings of maize.
We could see that in the youngest tissues examined by us the etioplast greening is very fast and occurs according to a particular pattern which is characterized by the contemporary presence of grana and large non crystalline prolamellar bodies. On the contrary, in the oldest examined tissues the etioplast greening is slow and the formation of grana appears to be delayed and subsequent to the using up of the prolamellar bodies.
In the young tissues the intensity of the light mainly affects the duration of the lag-phase preceding the chlorophyll accumulation, while in the old tissues it also affects the total amount of chlorophyllous pigments, the restraining effect of the light appearing amplified by a concomitant restraining effect of cell age.
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References
Axelsson, L., 1976a: The photostability of different chlorophyll forms in dark grown leaves of wheat. I. Stability to high intensity red light of forms appearing after photoreduction of protochlorophyllide. Physiol. Plant.38, 327–332.
—, 1976b: The photostability of different chlorophyll forms in dark grown leaves of wheat. II. Reaction kinetics for the photodecomposition of the 664-form and 673-form, Physiol. Plant.38, 333–336.
—, 1977: The photostability of different chlorophyll forms in dark grown leaves of wheat. III. Dependence on age of the plants. Physiol. Plant.41, 217–222.
Boffey, S. A., Sellden, G., Leech, R. M., 1980: Influence of cell age on chlorophyll formation in light-grown and etiolated wheat seedlings. Plant Physiol.65, 680–684.
Bradbeer, J. W., Gyldenholm, A. O., Ireland, H. M. M., Smith, J. W., Rest, J., Edge, H. J. W., 1974: Plastid development in primary leaves ofPhaseolus vulgaris. VIII. The effects of the transfer of dark-grown plants to continuous illumination. New Phytol.73, 271–279.
—,Arron, G. P., Herrera, A., Kemble, R. J., Montes, G., Sherrat, D., Wara-Aswapati, O., 1977: The greening of leaves. In: Integration of activity in higher plants, p. 195 (Symposia of the Society for Experimental Biology, SymposiumXXXI). Cambridge: Cambridge University Press.
—, 1981: Development of photosynthetic function during chloroplast biogenesis. In: Vol. 8, Photosynthesis, p. 424. The Biochemistry of Plants (Hatch, M. D., Boardman, N. K., eds.). New York-London-Toronto: Academic Press.
Brangeon, J., 1973: Compared ontogeny of the two types of chloroplasts ofZea mays. J. Microscopie16, 233–242.
Casadoro, G., Rascio, N., 1979: Pattern of thylakoid system formation. J. Ultrastruct Res.60 307–315
Farineau, N., Rousseaux, J., 1975: Influence de la 6-benzylaminopurine sur la différenciation plastidiale dans les cotylédons de cocombre. Physiol. Plant.33, 194–202.
Girnth, C., Bergfeld, R., Kasemir, H., 1978: Phytochromemediated control of grana and stroma thylakoid formation in plastids of mustard cotyledons. Planta141, 191–198.
Grumbach, K. H., Lichtenthaler, H. K., 1982: Chloroplast pigments and their biosynthesis in relation to light intensity. Photochem. Photobiol.35, 209–212.
Huffaker, R. C., Obendorf, R. L., Keller, C. J., Kleinkopf, G. E., 1966: Effects of light intensity on photosynthetic carboxilase phase enzymes and chlorophyll synthesis in greening leaves ofHordeum vulgare L. Plant Physiol.41, 913–918.
Kirichenko, E. B., Smolygina, L. D., Sedyuk, O. P., Vasil'eva, V. T., 1976: Synthesis and accumulation of pigments during development of the lamellar system of dimorphic plastids inZea mays L. Soviet. Plant Physiol.23, 18–22.
Kirk, J. T. O., Tilney-Basset, R. A. E., 1978: The plastids. Amsterdam-New York-Oxford: Elsevier/North-Holland Biomedical Press.
Kohn, S., Klein, S., 1976: Light-induced structural changes during incubation of isolated maize etioplasts. Planta132, 169–175.
Lancer, H. A., Cohen, C. E., Schiff, J. A., 1976: Changing ratios of phototransformable Protochlorophyll and Protochlorophyllide of bean seedlings developing in the dark. Plant Physiol.57, 369–374.
Maclachlan, S., Zalik, S., 1963: Plastid structure, chlorophyll concentration, and free amino acid composition of a chlorophyll mutant of barley. Can. J. Bot.41, 1053–1062.
Manetas, Y., Akoyunoglou, G., 1980: The conversion of δ-aminolevulinic acid-induced protochlorophyll(ide) to chlorophyll(ide) and its incorporation into the developing thylakoid. Photosynthetica14, 32–39.
Montes, G.,Bradbeer, J. W., 1974: The biogenesis of the photosynthetic membranes and the development of photosynthesis in greening maize leaves. Proceedings 3rd Internation. Congress on Photosynthesis (Avron, M., ed.), pp. 1867–1876.
Obendorf, R. L., Huffaker, R. C., 1970: Influence of age and illumination on distribution of several Calvin cycle enzymes in greening barley leaves. Plant Physiol.45, 579–582.
Rascio, N., Orsenigo, M., Arboit, D., 1976: Prolamellar body transformation with increasing cell age in the maize leaf. Protoplasma90, 253–263.
—,Casadoro, G., Di Chio, L., 1979: Etioplast-chloroplast transformation in sunflower cotyledons. Protoplasma100, 45–52.
—,Mariani, P., Orsenigo, M., 1980: The ultrastructural development of plastids in leaves of maize plants exposed to continuous illumination. Protoplasma102, 131–139.
Raven, C. W., 1973: Chlorophyll formation and phytochrome. Meded Landbouwhogesch. Wageningen73, 1–100.
Robards, A. W., 1981: General and Molecular Cytology. Progress in Botany43, 1–12. Berlin-Heidelberg-New York: Springer.
Robertson, D., McMillan Laetsch, W., 1974: Structure and function of developing barley plastids. Plant Physiol.54, 148–159.
Sestak, Z., 1977: Photosynthesis characteristics during ontogenesis of leaves I. Chlorophylls. Photosynthetica11, 367–448. accumulation of protochlorophyllide in leaves of different ages treated with δ-aminolevulinic acid. Plant Sci. Letters4, 89–96.
—,Bjorn, L. O., Virgin, H. I., 1980: Factors in chloroplast differentiation. In: Chloroplasts (Reinert, J., ed.), p. 201. Berlin-Heidelberg-New York: Springer.
Thomas, H., Stoddart, J. L., 1980: Leaf senescence. Ann. Rev. Plant Physiol.31, 83–111.
Treffry, T., 1978: Biogenesis of the photochemical apparatus. Int. Rev. Cytol.52, 159–196.
Virgin, H. I., 1955: Protochlorophyll formation and greening in etiolated barley leaves. Physiol. Plant.8, 630–643.
—, 1961: On the formation of Protochlorophyll in normal green wheat leaves of varying age. Physiol. Plant.14, 384–392.
—, 1972: Chlorophyll biosynthesis and phytochrome action. In: Phytochrome (Mitrakos, K., Shropshire, W., Jr., eds.), p. 372. London-New York: Academic Press.
Wellburn, A. R., Robinson, D. C., Wellburn, F. A. M., 1982: Chloroplast development in low light-grown barley seedlings. Planta154, 259–265.
Wild, A., Belz, J., Ruhle, W., 1981: Cyclic and noncyclic photophosphorylation during the ontogenesis of high-light and low-light leaves ofSinapis alba. Planta153, 308–311.
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Rascio, N., Mariani, P. & Casadoro, G. Etioplast-chloroplast transformation in maize leaves: Effects of tissue age and light intensity. Protoplasma 119, 110–120 (1984). https://doi.org/10.1007/BF01287823
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DOI: https://doi.org/10.1007/BF01287823