Summary
A detailed account of the ultrastructure and behaviour of the spindle pole body (SPB) of the entomophthoraceous fungusErynia neoaphidis is presented for the first time.
The SPB consists of extranuclear (ENC) and intranuclear (INC) components. The ENC is a “saucepan-shaped” structure which lies in a pocket of the nuclear envelope. It is composed of a forked, fibrillar “handle” and a shallow, cylindrical “pan”. The “pan” has a wall of two layers, both of which are thickened with a regular periodicity so that they appear to be “beaded”. It is postulated that the “pan“ is formed from rough endoplasmic reticulum and that it synthesizes the amorphous, electron-dense material coating the ENC.
The INC is a “saucer-shaped”, electron-dense plaque in which the ends of the spindle microtubules terminate. During metaphase, a “clear zone” separates the INC from the nuclear envelope and persists until telophase. The roles of the amorphous, electron-dense material and the “clear zone” as well as the method of SPB replication are discussed.
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References
Aist, J. R., Berns, M. W., 1981: Mechanics of chromosome separation during mitosis inFusarium (Fungi imperfecti): New evidence from ultrastructural and laser microbeam experiments. J. Cell Biol.91, 446–458.
Beckett, A., 1981: Ultrastructure and behaviour of nuclei and associated structures within the meiotic cells ofEuascomycetes. In: The Fungal Nucleus (Gull, K., Oliver, S. G., eds.). pp. 37–61. Cambridge: Cambridge University Press.
—,Crawford, R. M., 1970: Nuclear behaviour and ascospore delimitation inXylosphaera polymorpha. J. gen. Microbiol.63, 269–380.
—,Heath, I. B., McLaughlin, D. J., 1974: An atlas of fungal ultrastructure. London: Longman.
Ben Ze'ev, I., Kenneth, G. R., 1982: Features-criteria of taxonomic value in theEntomophthorales: I. A revision of the Batkoan classification. Mycotaxon14, 393–455.
Bland, C. E., Lunney, C. Z., 1975: Mitotic apparatus ofPilobolus crystallinus. Cytobiologie11, 382–391.
Butt, T. M., Beckett, A., 1984: Ultrastructure of mitosis in the aphid-pathogenic fungusErynia neoaphidis. Protoplasma120, 61–71.
Dustin, P., 1978: Microtubules. Berlin-Heidelberg-New York: Springer.
Dyson, R. D., 1978: Essentials of cell biology, 2nd ed. Boston-London-Sydney-Toronto: Allyn and Bacon Inc.
Franke, W. W., Reau, R., 1973: The mitotic apparatus of the Zygomycete,Phycomyces blakesleeanus. Arch. Microbiol.90, 120–129.
Gull, K., 1981: Microtubules and microtubule proteins in fungi. In: The Fungal Nucleus (Gull, K., Oliver, S. G., eds.), pp. 111–132. Cambridge: Cambridge University Press.
Gustafsson, M., 1971: Microbial control of aphids and scale insects. In: The microbial control of insects and mites (Burges, H. D., Hussey, N. W., eds.), pp. 375–384. London-New York: Academic Press.
Heath, I. B., 1978: Experimental studies of mitosis in the fungi. In: Nuclear division in the fungi (Heath, I. B., ed.), pp. 89–176. New York-San Francisco-London: Academic Press.
—, 1980: Variant mitosis in lower eukaryotes: Indicators of the evolution of mitosis? Int. Rev. Cytol.64, 1–80.
—, 1981: Nucleus-associated organelles in fungi. Int. Rev. Cytol.69, 191–220.
—,Heath, M. C., 1976: Ultrastructure of mitosis in the cowpea rust fungusUromyces phaseoli var.vignae. J. Cell Biol.70, 592–607.
—,Rethoret, K., 1982: Mitosis in the fungusZygorhynchus moelleri: Evidence for stage specific enhancement of microtubule preservation by freeze substitution. European J. Cell Biol.28, 180–189.
Howard, R. J., Aist, J. R., 1979: Hyphal tip cell ultrastructure of the fungusFusarium: Improved preservation by freeze-substitution. J. Ultrastruct. Res.66, 224–234.
Humber, R. A., 1975: Aspects of the biology of an insect-parasitic fungus,Strongwellsea magna (Zygomycetes: Entomophthorales). Ph.D. dissertation, University of Washington, Seattle.
— 1981: An alternative view of certain taxonomic criteria used in theEntomophthorales (Zygomycetes). Mycotaxon13, 191–240.
—, 1982:Strongwellsea vsErynia: The case for a phylogenetic classification of theEntomophthorales (Zygomycetes). Mycotaxon15, 167–184.
Luduena, R. F., 1979: Biochemistry of tubulin. In: Microtubules (Roberts, K., Hyams, J. S., eds.), pp. 65–116. New York: Academic Press.
McCully, E. K., Robinow, C. F., 1973: Mitosis inMucor hiemalis. Arch. Microbiol.94, 133–148.
McLaughlin, D. J., 1971: Centrosomes and microtubules during meiosis in the mushroomsBoletus rubinellus. J. Cell Biol.50, 737–745.
—, 1981: The spindle pole body and postmeiotic mitosis inAuricularia fuscosuccinea.59, 1196–1206.
Moorman, G. W., 1976: Mitosis inAncylistes. Mycologia68, 902–909.
Murrin, F., Heath, I. B., 1982: Mithramycin staining of condensed fungal chromatin. Experimental Mycology6, 303–306.
O'Donnell, K. L., McLaughlin, D. J., 1981: Ultrastructure of mitosis in the hollyhock rust fungusPuccinia malvacearum I. Prophase I—Prometaphase I. Protoplasma108, 225–244.
Patterson, D. J., 1980: Contractile vacuoles and associated structures: their organization and function. Biol. Rev.55, 1–46.
Peabody, D. C., Motta, J. J., 1979: The ultrastructure of nuclear division inArmillaria mellea: meiosis I. Can. J. Bot.57, 1860–1872.
Petzelt, C., 1979: Biochemistry of the mitotic spindle. Int. Rev. Cytol.60, 53–92.
Pickett-Heaps, J. D., 1969: The evolution of the mitotic apparatus: an attempt at comparative ultrastructural cytology in dividing plant cells. Cytobios1, 257–280.
Pickett-Heaps, J. D., Tippitt, D. H., 1978: The diatom spindle in perspective. Cell14, 455–467.
Raff, E., 1979: Control of microtubule assemblyin vivo. Int. Rev. Cytol.59, 1–96.
Remaudière, G., Keller, S., 1980: Revision systematique des genres d'Entomophthoraceae a potentialite entomopathogene. Mycotaxon11, 323–338.
Reynolds, E. S., 1963: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol.17, 208–212.
Setliff, E. C., Hoch, H. C., Patton, R. F., 1974: Studies on nuclear division in basidia ofPoria latemarginata. Can. J. Bot.52, 2323–2333.
Spiegel, F. W., 1982: Mitosis in the protostelidPlanoprotostelium aurantium. Protoplasma113, 178–188.
Wilding, N., 1969: Effect of humidity on sporulation ofEntomophthora aphidis andE. thaxteriana. Trans. Brit. mycol. Soc.53, 126–130.
Zickler, D., 1970: Division spindle and centrosomal plaques during mitosis in some Ascomycetes. Chromosoma, Berlin30, 287–304.
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Butt, T.M., Beckett, A. Ultrastructure and behaviour of the spindle pole body of the aphid-pathogenic fungusErynia neoaphidis . Protoplasma 120, 61–71 (1984). https://doi.org/10.1007/BF01287618
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DOI: https://doi.org/10.1007/BF01287618