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Division polarity, development and configuration of microtubule arrays in Bryophyte meiosis

I. Meiotic prophase to metaphase I

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Summary

Immunofluorescence and TEM studies of meiosis in two mosses (Bryophyta) provide evidence that the prophasic tetrahedral system of microtubules contributes directly to the metaphase I spindle. Intense staining of tubulin, conspicuously absent around the nuclear envelope, is first seen associated with plastids. By mid-prophase, microtubules radiate from the plastids to the nuclear envelope and become organized into six bands that interconnect the four plastids, forming a tetrahedral cytoskeleton surrounding the nucleus. During transition of prophase to metaphase, the four poles of the tetrahedral microtubule system converge in pairs toward opposite cleavage furrows. Opposite furrows occupy mutually perpendicular planes and the pair of microtubule focal points straddling one furrow lies at right angles to the pair straddling the opposite furrow. Additional microtubules terminate in numerous small clusters in the concave polar regions arching over the cleavage furrows. By early anaphase, the microtubule focal points lie very close to the division axis. We conclude that microtubules recruited from the prophasic quadripolar system are incorporated into the mature metaphase I spindle and the two principal focal points at each pole are those derived from poles of the prophasic quadripolar system.

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  1. Department of Biology, The University of Southwestern Lousiana, 70504-2451, Lafayette, LA, USA

    R. C. Brown & B. E. Lemmon

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  1. R. C. Brown
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  2. B. E. Lemmon
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Brown, R.C., Lemmon, B.E. Division polarity, development and configuration of microtubule arrays in Bryophyte meiosis. Protoplasma 137, 84–99 (1987). https://doi.org/10.1007/BF01281144

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