Summary
Two distinguishable populations of microfilaments (mfs) can be identified in the radish root hair. Bundles of mfs are found throughout the cytoplasm, excluding the tip region of the hair. Single mfs occur only as a cortical array, specifically associated with the microtubules. Both mf populations are oriented parallel to the direction of streaming. Hairs grown in 5 μg/ml cytochalasin B (CB) exhibit site-specific differential responses to the drug in both their streaming pattern and sensitivity of their mfs. Cytochalasin B elicits the following responses: 1. cytoplasmic streaming is reduced in all regions of the hair; 2. small particles (<1 μm in diameter) still stream, whereas large particles (>1 μm in diameter) no longer stream but exhibit an oscillatory or rotational motion; 3. filament bundles show increasing sensitivity to CB along the length of the hair; 4. single mfs show decreasing sensitivity to CB along the hair length. The effects of CB on cytoplasmic streaming can be related to its effects on both mf populations, thus suggesting that although mf bundles are probably involved in streaming in the sub apical and basal regions of the hair, single mfs are most likely involved in generating the slower, more irregular streaming patterns exhibited in the hair tip and CB-treated hair base.
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References
Bradley, M. O., 1973: Microfilaments and cytoplasmic streaming: inhibition of streaming with cytochalasin. J. Cell Sci.12, 327–343.
Cande, W. Z., Goldsmith, M. H., Ray, P. M., 1973: Polar auxin transport and auxin induced elongation in the absence of cytoplasmic streaming. Planta111, 279–296.
Chen, J. C. W., 1973: Observations of protoplasmic behaviour and motile protoplasmic fibrils in cytochalasin B treatedNitella rhizoid. Protoplasma77, 427–435.
Hepler, P. K., Palevitz, B. A., 1974: Microtubules and microfilaments. Ann. Rev. Plant Physiol.25, 309–362.
Mascarenhas, J. P., la Fountain, J. R., 1972: Protoplasmic streaming, cytochalasin B, and growth of the pollen tube. Tissue and Cell4, 11–14.
Maupin-Szamier, I. P., Pollard, T. D., 1978: Actin filament destruction by osmium tetroxide. J. Cell Biol.77, 837–852.
Nagai, R., Kamiya, N., 1977: Differential treatment ofChara cells with cytochalasin B with specific reference to its effect on cytoplasmic streaming. Expt. Cell Res.108, 231–237.
O'Brien, T. P., McCully, M. E., 1970: Cytoplasmic fibers associated with streaming and saltatory particle movement inHeracleum mantegazzianum. Planta94, 91–94.
Pollard, T. D., 1975: Functional implications of the biochemical and structural properties of cytoplasmic contractile proteins. In: Molecules and cell movement (Inoué, S., Stephens, R. E., eds.), pp. 259–286. New York: Raven Press.
—,Maupin-Szamier, I. P., 1978: Electron microscopy of cytoplasmic contractile proteins. 9th International Congress on Electron Microscopy, Vol. III, pp. 606–614. Toronto: Microscopical Society of Canada.
Seagull, R. W., Heath, I. B., 1979: The effects of tannic acid on thein vivo preservation of microfilaments. European J. Cell Biol.20, 184–188.
— —, 1980: The organization of cortical microtubule arrays in the radish root hair. Protoplasma103, 205–229.
Wessells, N. K., Spooner, B. S., Ash, J. F., Bradley, M. D., Luduena, M. A., Taylor, E. L., Wrenn, J. T., Yaniada, K. M., 1971: Microfilaments in cellular and developmental processes. Science171, 135–143.
Williamson, R. E., 1972: A light microscope study of the action of cytochalasin B on the cells and isolated cytoplasm ofCharaceae. J. Cell Sci.10, 811–819.
—, 1975: Cytoplasmic streaming inChara: a cell model activated by ATP and inhibited by cytochalasin B. J. Cell Sci.17, 655–668.
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Seagull, R.W., Heath, I.B. The differential effects of cytochalasin B on microfilament populations and cytoplasmic streaming. Protoplasma 103, 231–240 (1980). https://doi.org/10.1007/BF01276269
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DOI: https://doi.org/10.1007/BF01276269