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The dynamic behavior of cytoplasmic F-actin in growing hyphae

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Summary

A dynamic population of cytoplasmic F-actin was observed with electroporated rhodamine phalloidin (RP) staining in growing hyphae ofSaprolegnia ferax. This central actin population was distinct from the fibrillar peripheral network previously described in chemically fixed hyphae in that it was diffuse, pervaded the entire cytoplasm and was most concentrated in the central cytoplasm 8.4 μm from the tip. The peripheral network did not stain with electroporated RP. The apical concentration of central cytoplasmic actin was only present in growing hyphae and developed prior to tip extension. It co-localized with the polarized distribution of mitochondria and endoplasmic reticulum in the tip, suggesting that it functions in positioning these organelles during tip growth. Within the central actin there was a consistent apical cleft which only occurred in growing hyphae and whose position predicted the direction of tip growth. This cleft was coincident with the known accumulation of apical wall vesicles, suggesting that it is either established by vesicle exclusion of the central actin network or is permeated by a portion of the in vivo unstained peripheral network. Photobleaching studies showed that in both growing and non-growing hyphae, cytoplasmic actin continually and rapidly moved from subapical regions to the tip where it accumulated. It mostly moved forward at the rate of tip growth, while some also left the tip, presumably to populate subapical regions.

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Abbreviations

RP:

rhodamine phalloidin

F-actin:

filamentous actin

DIC:

Nomarski differential interference contrast

FITC:

fluorescein isothiocyanate

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Author notes

  1. S. L. Jackson

    Present address: Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, G.P.O. Box 475, 2601, Canberra City, ACT, Australia

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  1. Biology Department, York University, North York, Ontario

    S. L. Jackson & I. B. Heath

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  1. S. L. Jackson
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  2. I. B. Heath
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Jackson, S.L., Heath, I.B. The dynamic behavior of cytoplasmic F-actin in growing hyphae. Protoplasma 173, 23–34 (1993). https://doi.org/10.1007/BF01378859

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  • DOI: https://doi.org/10.1007/BF01378859

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