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Differential cell wall degradation byErwinia chrysanthemi in petiole ofSaintpaulia ionantha

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Summary

Erwinia chrysanthemi is a soft-rot pathogenic enterobacterium that provokes maceration of host plant tissues by producing extracellular cell-wall-degrading enzymes, among which are pectate lyases, pectin methyl esterases, and cellulases. Cell wall degradation in leaves and petiole tissue of infectedSaintpaulia ionantha plants has been investigated in order to define the structural and temporal framework of wall deconstruction. The degradation of major cell wall components, pectins and cellulose, was studied by both classical histochemical techniques (Calcofluor and periodic acid-thiocarbohydrazide-silver proteinate staining) and immunocytochemistry (tissue printing for detection of pectate lyases; monoclonal antibodies JIM5 and JIM7 for detection of pectic substrates). The results show that the mode of progression of the bacteria within the host plant is via the intercellular spaces of the parenchyma leaf and the petiole cortex. Maceration symptoms and secretion of pectate lyases PelA, -D, and -E can be directly correlated to the spread of the bacteria. Wall degradation is very heterogeneous. Loss of reactivity with JIM5 and JIM7 was progressive and/or clearcut. The primary and middle lamella appear to be the most susceptible regions of the wall. The innermost layer of the cell wall frequently resists complete deconstruction. At the wall intersects and around intercellular spaces resistant domains and highly degraded domains occurred simultaneously. All results lead to the hypothesis that both spatial organisation of the wall and accessibility to enzymes are very highly variable according to regions. The use of mutants lacking pectate lyases PelA, -D, -E or -B, -C confirm the important role that PelA, PelD, and PelE play in the rapid degradation of pectins from the host cell walls. In contrast, PelB and PelC seem not essential for degradation of the wall, though they can be detected in leaves infected with wild-type bacteria. With Calcofluor staining, regularly localised cellulose-rich and cellulose-poor domains were observed in pectic-deprived walls.

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Abbreviations

MAb:

monoclonal antibody

PATAg:

periodic acid-thiocarbohydrazide-silver proteinate

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Authors and Affiliations

  1. Laboratoire de Pathologie Végétale, Institut National Agronomique, 16 rue Claude Bernard, F-75231, Paris Cedex 05, France

    L. Murdoch, J. C. Corbel, D. Reis, Y. Bertheau & B. Vian

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  1. L. Murdoch
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  2. J. C. Corbel
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  3. D. Reis
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  4. Y. Bertheau
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  5. B. Vian
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Murdoch, L., Corbel, J.C., Reis, D. et al. Differential cell wall degradation byErwinia chrysanthemi in petiole ofSaintpaulia ionantha . Protoplasma 210, 59–74 (1999). https://doi.org/10.1007/BF01314956

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  • DOI: https://doi.org/10.1007/BF01314956

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