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The effect of β-adrenergic blockade on infarct size following experimental coronary occlusion

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Summary

The effect of Pindolol on myocardial infarct size was studied in 10 open chest dogs. In each animal a sequential occlusion and reperfusion of 2 medium-sized branches of the left coronary artery was performed in the same heart. After occlusion and reperfusion of the control artery the initial dose of Pindolol (0.25 mg/kg body weight) was administered. Thereafter the test artery was occluded, followed by a maintenance dose of Pindolol (0.3 mg/kg body weight).

The drug caused a significant decrease in LVP and LV-dp/dt but no change in heart rate. MVO2 also decreased significantly. Regional myocardial blood flow was measured with the tracer microsphere method. Collateral flow in the perfusion area of the control artery was 11.2±5.9% and in the area of the test artery 10.0±4.4% of normal. No change in the endo/epi ratio as a result of treatment was observed.

The area of infarction (p-nitroblue tetrazolium-reaction) was divided by the area of perfusion (angiography). Infarct size, expressed as the percentage of the perfusion area. was 48.2±22.2% in the region of the control artery and 43.0±23.9% in the region of the test artery. The difference was statistically not significant.

Zusammenfassung

An 10 Hunden wurde die Wirkung von Pindolol auf die Hämodynamik, die regionale Myokardperfusion und die Infarktgröße nach experimentellem Koronarverschluß untersucht. An jedem Herzen wurden hintereinander 2 mittelgroße Äste der linken Koronararterie okkludiert. Nach Ligatur (90 Minuten) und Reperfusion der ersten Arterie (Kontrollarterie) wurde die Inititaldosis Pindolol (0,25 mg/kg Körpergewicht) infundiert. Während der Ligatur (90 Minuten) der zweiten Arterie (Testarterie) wurde eine Erhaltungsdosis Pindolol (0,3 mg/kg Körpergewicht/90 Minuten) infundiert. Der linke Ventrikeldruck, LV-dp/dt max., Aortendruck und Herzfrequenz wurden kontinuierlich gemessen. Der myokardiale Sauerstoffverbrauch wurde vom Computer (Bretschneider-Formel) während des Experimentes berechnet. Die Myokarddurchblutung wurde mit Hilfe der “tracer microsphere”-Technik bestimmt. Das Nekrosegebiet wurde makrohistochemisch (NBT-Färbung) und das Perfusionsgebiet der verschlossenen Arterie wurde angiographisch definiert. Die resultierende Infarktgröße wurde als Quotient aus Nekrose-und Perfusionsgebiet in Prozent ausgedrückt. Pindolol verursachte einen signifikanten Abfall des linken Ventrikeldrucks, und LV-dp/dt, die Herzfrequenz, änderte sich nicht. Der myokardiale Sauerstoffverbrauch nahm signifikant von 7,9±1,4 auf 6,9±1,9 ml/min×100g ab. Der Kollateralfluß betrug im Perfusionsgebiet der Kontrollarterie 11,2±5,9% und in dem der Testarterie 10,0±4,4% der Normaldurchblutung. Die Infarktgröße konnte durch Pindolol nicht beeinflußt werden, sie betrug nach Verschluß der Kontrollarterie 48,2±22,2% und nach Verschluß der Testarterie 43,0±23,9%.

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Author notes

  1. K. Genth

    Present address: Department of Experimental Cardiology, Max-Planck-Institute for Physiological and Clinical Research, Bad Nauheim, West Germany

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  1. Department of Experimental Cardiology, Max-Planck-Institute for Physiological and Clinical Research, Bad Nauheim, West Germany

    M. Hofmann, Mechthild Hofmann & W. Schaper

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Genth, K., Hofmann, M., Hofmann, M. et al. The effect of β-adrenergic blockade on infarct size following experimental coronary occlusion. Basic Res Cardiol 76, 144–151 (1981). https://doi.org/10.1007/BF01907953

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