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Alterations in heart sarcolemmal Ca2+-ATPase and Ca2+-binding activities due to oxygen free radicals

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Summary

Effects of oxygen free radicals on Ca2+/Mg2+ ATPase and ATP-independent Ca2+-binding activities were examined in rat heart sarcolemma. Membranes were incubated with different oxygen radical generating media such as xanthine + xanthine oxidase, hydrogen peroxide, and hydrogen peroxide + Fe2+. In the presence of xanthine + xanthine oxidase, Ca2+ ATPase activity was stimulated and this effect was prevented by the addition of superoxide dismutase. Hydrogen peroxide also showed a significant increase in Ca2+-ATPase activity in a dose-dependent manner and this effect was blocked by catalase. On the other hand, a combination of hydrogen peroxide + Fe2+ decreased Ca2+-ATPase activity; this depression was prevented by the addition of D-mannitol. The observed change in Ca2+-ATPase activity due to oxygen free radicals was associated with changes in Vmax, whereas Ka remained unaffected. Both xanthine + xanthine oxidase and hydrogen peroxide increased whereas, hydrogen peroxide + Fe2+ inhibited the ATP-independent Ca2+-binding activities. It is suggested that oxygen free radicals may influence Ca2+ movements in the cell by altering the Ca2+/Mg2+ ATPase and Ca2+-binding activities of the membrane and these effects may be oxygen-radical species specific.

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Author notes

  1. N. S. Dhalla

    Present address: Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada

Authors and Affiliations

  1. Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada

    M. Kaneko & P. K. Singal

  2. Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada

    M. Kaneko, P. K. Singal &  N. S. Dhalla

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  1. M. Kaneko
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  2. P. K. Singal
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  3. N. S. Dhalla
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Kaneko, M., Singal, P.K. & Dhalla, N.S. Alterations in heart sarcolemmal Ca2+-ATPase and Ca2+-binding activities due to oxygen free radicals. Basic Res Cardiol 85, 45–54 (1990). https://doi.org/10.1007/BF01907013

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  • DOI: https://doi.org/10.1007/BF01907013

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