Abstract
The enzymatic hydrolysis of a model poly(D, L-lactide) by cutinase was studied by using a barostat surface balance. A theoretical approach based on the adaptation of the Michaelis-Menten scheme at the interface and a process of random fragmentation of the macromolecules was developed. The role of the interfacial organisation of the reaction products was discussed. Hydrolysis rate constant values and the specific activity were estimated and compared with those obtained for the hydrolysis of lipid monolayers. The process of fragmentation of the interfacial polymer structures was visualised by using AFM imaging.
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Lewis DD (1990) In: Chasin M, Langer RS (eds) Biodegradable Polymer as Drug Delivery Systems. Marcel Dekker, New York, p 1
Vert M (1986) Buck S (eds) CRC Critical Review — Therapeutic Drug Carrier systems, Vol. 2. CRC Press, Boca Raton, p291
Holand SJ, Tighe BJ, Gould PL (1986) J Control Release 4:155
Mauduit J, Vert M (1993) STP Pharma Sci 3(3): 197
Chu CC (1982) J Biomed Mater Res 16:117
Makino K, Arakawa M, Kondo T(1985) Chem Pharm Bull 33:1195
Makino K, Ohshima H, Kondo T (1986) J Microencapsulation 3:203
Coffin MD, McGinity W (1992) Pharm Res 9:200
Chu CC, Williams DF (1983) J Biomed Mater Res 17:1029
Kaetsu I, Yoshida M, Asano M, Yamanaka H, Imai K, Yuasa H, Mashimo T, Suzuki K, Katakai R, Oya M (1987) J Control Release 6:249
Vert M, Li S, Garreau H (1991) J Control Res 16:15
Fukuzaki H, Yoshida M, Asano M, Kumakura M, Mashimo T, Yuasa H, Imai K, Yamanaka H (1991) Biomaterials 12:433
Spenlehauer G, Vert M, Benoit JP, Boddaert A (1989) Biomaterials 10:557
Göpferich A (1996) Biomaterials 17:103
Shih C (1995) J Control Release 34:9
Ivanova Tz, Panaiotov I, Boury F, Proust JE, Benoit JP, Verger R (in press) Coll Interface Sci B: Biointerfaces, in press
Vert M. Christel P, Chabot F, Leray J (1984) In: Hasting G, Wand Ducheyne P (eds) CRC Press, Boca Raton, p 119
Lauwereys M, De Geus P, De Meutter J, Stanssen P, Mattyssens G (1991) In: Alberghina L et al (eds) Lipases-Structure, Mechanism and Genetic Engineering, Vol. 16 VCH, Weinheim, p 243
Verger R, de Haas G (1973) Chem Phys Lipids 10:127
Boury F, Olivier E, Proust JE, Benoit JP (1993 & 1994) J Colloid Interface Sci 160:1; 163:37
Boury F, Gulik A, Dedieu JC, Proust JE (1994) Langmuir 10:1654
Boury F, Ivanova Tz, Panaiotov I, Proust JE, Bois A, Richou J (1995) J Colloid Interface Sci 169:380 (1995) Langmuir 11:1636
Verger R, Mieras MC, de Haas GH (1973) J Biol Chem 248:4023
Havsteen BH, Varon Castellanos R, Molina M, Garcia Meseguer JM, Valero E, Garcia-Moreno M (1992) J Theor Biol 157:523
Zografi G, Verger R, de Haas GH (1971) Chem Phys Lipids 7:185
Vert M, private communication
Egmond MR (1996) In: Malcata FX (ed) Engineering of/with Lipases NATO AS I Series E: Applied Scdiences, Vol 317. Kluwer Acad Publ, (Dordrecht, pp 183 and 193
Melo E, Ivanova M, Aires-Barros M, Gabral J, Verger R (1995) Biochemistry 34:1615
Ivanova M, Ivanova Tz, Verger R, Panaiotov I (1996) Coll Surface B: Bioint 6:9
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Ivanova, T.Z., Panaiotov, I., Boury, F. et al. Enzymatic hydrolysis of poly(D, L-lactide) spread monolayers by cutinase. Colloid Polym Sci 275, 449–457 (1997). https://doi.org/10.1007/s003960050103
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DOI: https://doi.org/10.1007/s003960050103