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Experimental focal cerebral ischaemia in rabbits

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Summary

A highly reproducible form of experimental embolization of the intracranial arteries is presented in rabbits. The injection of a silver or gold ball into the internal carotid artery caused occlusion predominantly of the middle cerebral artery and/or its branches. At the moment when embolization took place, the characteristic signs of acute cerebral ischaemia occurred in the electroencephalogram, local cerebral blood flow and steady (DC) potentials. Several hours after the ball had been injected the extent of the focal lesions became recognizable on sections stained for myelin. The procedure is simple, rapid, inexpensive and practically always successful. The extent of the lesion may be influenced by the change of both the ball size and the posture of animals. Moreover, the site of occlusion is easily discernible both on radiographs and to the naked eye.

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References

  1. Blair RDG, Waltz AG (1970) Regional cerebral blood flow during acute ischemia. Neurology (Minneap) 20:802–808

    Google Scholar 

  2. Bremer AM, Watanabe O, Bourke RS (1975) Arteficial embolization of the middle cerebral artery in primates. Stroke 6:387–390

    Google Scholar 

  3. Diaz FG, Mastri AR, Ausman JI, Chou SN (1979) Acute cerebral revascularization. I. Cerebral ischemia experimental animal model. Surg Neurol 13:353–362

    Google Scholar 

  4. Garcia JH (1984) Experimental ischemic stroke: a review. Stroke 15:5–14

    Google Scholar 

  5. Garcia JH, Kamijyo Y (1974) Cerebral infarction. Evolution of histopathological changes after occlusion of the middle cerebral artery in primates. J Neuropathol Exp Neurol 33:408–421

    Google Scholar 

  6. Handa J (1964) Experimental studies on the production and prevention of cerebral infarction in dogs. J Neuropathol Exp Neurol 23:457–476

    Google Scholar 

  7. Harvey K, Rasmussen I (1951) Occlusion of the middle cerebral artery. An experimental study. Arch Neurol Psychiatry 66:20–29

    Google Scholar 

  8. Heilbrun MP, Goldring S (1968) Steady potentials and pathologic correlates of cerebrovascular occlusion of dog. Arch Neurol 17:410–420

    Google Scholar 

  9. Heiss WD, Hayakawa T, Waltz A (1976) Patterns of changes of blood flow and relationships to infarction in experimental cerebral ischemia. Stroke 7:454–459

    Google Scholar 

  10. Hill ND, Millikan CH, Wakim KG (1955) Studies in cerebrovascular diseases. VII. Experimental production of cerebral infarction by intracarotid injection of homologous blood clot. Mayo Clin Proc 30:625–633

    Google Scholar 

  11. Hossmann KA (1971) Cortical steady potential, impedance and excitability changes during and after total ischemia of cat brain. Exp Neurol 32:163–175

    Google Scholar 

  12. Hubbard JL, Sundt TM jr (1983) Failure of naloxone to affect focal incomplete cerebral ischemia and collateral blood flow in cats. J Neurosurg 59:237–244

    Google Scholar 

  13. Hudgins WR, Carcia JH (1970) Transorbital approach to the middle cerebral artery of the squirrel monkey: a technique for experimental cerebral infarction applicable to ultra structural studies. Stroke 1:107–111

    Google Scholar 

  14. Ishihara N, Welch KMA, Meyer JS, Chabri E, Naritomi H, Wang TPF, Nell JH, Hsu MC, Miyakawa Y (1979) Influence of cerebral embolism on brain monoamines. J Neurol Neurosurg Psychiatry 42:847–853

    Google Scholar 

  15. Jeppson PG, Olin T (1960) Cerebral angiography in the rabbit. An investigation of vascular anatomy and variation in circulatory patterns with conditions of injection. Lunds Univ Arsskr NF Avd 2 56/14/:1–56

    Google Scholar 

  16. Kempinsky WH (1954) Steady potential gradients in experimental cerebral vascular occlusion. Electroencephalogr Clin Neurophysiol 6:375–388

    Google Scholar 

  17. Kudo M, Aoyama A, Ichimori S, Fukunaga N (1982) An animal model of cerebral infarction. Homologous blood clot emboli in rats. Stroke 13:505–508

    Google Scholar 

  18. Little JR, Sundt TM jr, Kerr FWL (1974) Neuronal alterations in developing cortical infarction. An experimental study in monkeys. J Neurosurg 39:186–198

    Google Scholar 

  19. McDonald DA, Potter JU (1951) The distribution of blood to the brain. J Physiol (Lond) 114:356–371

    Google Scholar 

  20. Masland RL, Netsky GM (1961) The electroencephalogram in experimental cerebral embolism. In: Meyer JS, Gastaut H (eds) Cerebral anoxia and the encephalogram. Charles C. Thomas, Springfield

    Google Scholar 

  21. Meyer JS (1958) Importance of ischemic damage to small vessels in experimental cerebral infarction. J Neuropathol Exp Neurol 17:571–585

    Google Scholar 

  22. Meyer JS (1958) Circulatory changes following occlusion of the middle cerebral artery and their relation to function. J Neurosurg 15:653–673

    Google Scholar 

  23. Meyer JS, Denny-Brown D (1955) Studies of cerebral circulation in brain injury. I. Validity of combined local cerebral electropolarography, thermometry and steady potentials as an indicator of focal circulatory and functional changes. Electroencephalogr Clin Neurophysiol 7:511–528

    Google Scholar 

  24. Meyer JS, Gotoh F, Tazaki Y (1962) Circulation and metabolism following experimental cerebral embolism. J Neuropathol Exp Neurol 21:4–24

    Google Scholar 

  25. Molinari GF (1970) Experimental cerebral infarction. I. Selective segmental occlusion of intracranial arteries in dog. Stroke 1:224–231

    Google Scholar 

  26. Molinari GF (1970) Experimental infarction. II. Clinicopathological model of deep cerebral infarcts. Stroke 1:232–244

    Google Scholar 

  27. Molinari GF, Laurent JP (1976) A classification of experimental models of brain ischemia. Stroke 7:14–17

    Google Scholar 

  28. O'Brien MD, Waltz AG (1973) Transorbital approach for occluding the middle cerebral artery without craniectomy. Stroke 4:201–206

    Google Scholar 

  29. Okada Y, Shima T, Yokoyama N, Uozumi T (1983) Comparison of middle cerebral artery trunk occlusion by silicone cylinder embolization and by trapping. J Neurosurg 58:492–499

    Google Scholar 

  30. Penry JK, Netsky MG (1960) Experimental embolic occlusion of a single leptomeningeal artery. Arch Neurol 3:391–398

    Google Scholar 

  31. Prolo DJ, Stilwell D (1962) Arterial supply to the mesencephalon and some associated areas of the rabbit brain. J Comp Neurol 119:229–254

    Google Scholar 

  32. Scremin OU, Sonnenschein RR, Rubinstein EH (1982) Cerebrovascular anatomy and blood flow measurement in the rabbit. J Cereb Blood Flow Metab 2:55–66

    Google Scholar 

  33. Seylaz J, Molnár L (1965) Nouvelle technique l'enregistrement synchrone du débit sanguin et de l'activité électrique du cerveau chez les animaux vigiles. CR Séances Soc Biol (Paris) 160:258–261

    Google Scholar 

  34. Steegmann AT, de la Fuente J (1959) Experimental cerebral embolism. II. Microembolism of the rabbit brain with seran polymer resin. J Neuropathol Exp Neurol 18:537–558

    Google Scholar 

  35. Sundt TM Jr, Waltz AG (1966) Experimental cerebral infarction: retroorbital, extradural approach for occluding the middle cerebral artery. Mayo Clin Proc 41:159–168

    Google Scholar 

  36. Swank RL, Hain RF (1952) The effect of different sized emboli on the vascular system and parenchyma of the brain. J Neuropathol Exp Neurol 11:280–299

    Google Scholar 

  37. Symon L, Ishikawa S, Meyer JS (1963) Cerebral arterial pressure changes and development of leptomeningeal collateral circulation. Neurology (Minneap) 13:237–250

    Google Scholar 

  38. Vander Eecken HM (1959) Anastomoses between the leptomeningeal arteries of the brain. Their morphological, pathological and clinical significance. Charles C. Thomas, Springfield

    Google Scholar 

  39. Villaret M, Cachera R (1939) Les embolies cérébrales. Masson, Paris

    Google Scholar 

  40. Winding O (1981) Cerebral microembolization following carotid injection of dextran microspheres in rabbits. Neuroradiology 21:123–126

    Google Scholar 

  41. Yonas H, Wolfson SK, Dujowny M, Boehuke M, Cook E (1981) Selective lenticulostriate occlusion in the primate. A highly focal cerebral ischemia model. Stroke 12:567–572

    Google Scholar 

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Authors and Affiliations

  1. Department of Neurology and Psychiatry, University Medical School of Debrecen, H-4012, Debrecen, Hungary

    K. Hegedüs, I. Fekete, F. Tury & L. Molnár

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  1. K. Hegedüs
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  2. I. Fekete
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  3. F. Tury
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  4. L. Molnár
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Hegedüs, K., Fekete, I., Tury, F. et al. Experimental focal cerebral ischaemia in rabbits. J Neurol 232, 223–230 (1985). https://doi.org/10.1007/BF00313784

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  • DOI: https://doi.org/10.1007/BF00313784

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