Summary
In 40 rabbits cerebral ischaemia was induced by autologous blood clot emboli injected into the middle cerebral artery. Autologous blood clot formed spontaneously (within 2 h) in a catheter fixed in the internal carotid artery. The effects of embolization were investigated on arterial blood pressure, respiration, local cerebral blood flow, locally available O2 (aO2), steady (DC) potentials and EEG. After embolization the blood pressure and the frequency of respiration decreased transiently. On the embolized side the local cerebral blood flow, aO2, the frequency and amplitude of the EEG diminished markedly and the DC potentials shifted to negative. Similar but less marked and short-lasting changes could be seen on the contralateral side. Pathological examination of the brains revealed extensive infarction with haemorrhagic components. The technique is a promising method for inducing stroke experimentally and offers various ways for its thorough investigation.
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References
Diaz FG, Mastri AR, Ausman JI, Chou SN (1979) Acute cerebral revascularization. I. Cerebral ischemia experimental animal model. Surg Neurol 12:353–362
Garcia JH (1984) Progress in cerebrosvascular disease. Experimental ischemic stroke: a review. Stroke 15:5–14
Hegedüs K, Fekete I, Tury F, Molnár L (1985) Experimental focal cerebral ischemia in rabbits. J Neurol 232:223–230
Heiss WD, Hayakowa T, Waltz AG (1976) Cortical neural function during ischemia. Arch Neurol 33:813–820
Hill ND, Millikan CH, Wakim KG (1955) Studies in cerebrovascular disease. VII. Experimental production of cerebral infarction by intracarotid injection of homologous blood clot. Mayo Clin Proc 30:625–633
Hudgins WR, Garcia JH (1970) Transorbital approach to the middle cerebral artery of the squirrel monkey: a technique for experimental cerebral infarction applicable to ultrastructural studies. Stroke 1:107–111
Hudgins WR, Garcia JH (1970) The effect of electrocautery, atmospheric exposure, and surgical retraction of the permeability of the blood-brain barrier. Stroke 1:375–380
Kaneko D, Nakamusa N, Ogawa T (1985) Cerebral infarction in rats using homologous blood emboli: development of a new experimental model. Stroke 16:76–84
Kudo M, Aoyama A, Schinari S, Tukunaga N (1982) An animal model of cerebral infarction homologous blood clot emboli in rats. Stroke 13:505–508
Lehmenkühler A (1979) Interrelationships between DC potentials, potassium activity, pO2 and pCO2 in the cerebral cortex of the rat. In: Speckmann EJ, Caspers H (eds) Origin of cerebral field potentials. Thieme, Stuttgart, pp 49–59
Masland RL, Netsky GM (1961) The electroencephalogram in experimental cerebral embolism. In: Meyer JS, Gastaut H (eds) Cerebral anoxia and the electroencephalogram. Thomas, Spring-field, pp 60–71
Meyer JS, Fang HC, Denny-Brown D (1954) Polygraphic study of cerebral collateral circulation. Arch Neurol 72:296–312
Molinari GF (1979) Clinical relevance of experimental stroke models. In: Price TR, Nelson E (eds) Cerebrovascular diseases. Eleventh Princeton Conference. Raven Press, New York, pp 19–34
Molinari GF, Laurent JP (1976) A classification of experimental models of brain ischemia. Stroke 7:14–17
Montgomery H, Horwitz O (1950) Oxygen tension of tissues by the polarographic method. J Clin Invest 29:1120–1130
Morawetz RB, DeGirolami U, Ojemann RG, Marcoux FW, Crowell RM (1978) Cerebral blood flow determined by hydrogen clearance during middle cerebral artery occlusion in unanesthetized monkeys. Stroke 9:143–149
Rumbaugh CL, Davis DO, Gilsin JM (1968) Experimental cerebral emboli: angiographic evaluation of autologous emboli in the dog. Invest Radiol 3:330–336
Schmidt CF (1950) The cerebral circulation in health and diseases. Thomas, Springfield
Scremin OU, Sonnenschein RR, Rubinstein EH (1982) Cerebrovascular anatomy and blood flow measurement in the rabbit. J Cereb Blood Flow Metab 2:55–66
Seylaz J, Molnár L (1965) Nouvelle technique pour l'enregistrement synchrone du débit sanguin et de l'activité électrique du cerveau chez les animaux vigiles. CR Séances Soc Biol (Paris) 160:258–261
Siesjö BK (1978) Brain energy metabolism. Wiley, Chichester
Sundt TM Jr, Waltz AG (1966) Experimental cerebral infarction: retroorbital, extradural approach for occulding the middle cerebral artery. Mayo Clin Proc 41:159–168
Waltz AG, Sundt TM (1967) The microvasculature and microcirculation of the cerebral cortex after arterial occlusion. Brain 90:681–697
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Fekete, I., Hegedüs, K. & Molnár, L. Cerebral ischaemia produced by homologous blood clot emboli in rabbit. J Neurol 235, 314–317 (1988). https://doi.org/10.1007/BF00314181
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DOI: https://doi.org/10.1007/BF00314181