Summary
The concentration of surface hydroxyl groups on three types of capillary columns was determined by exchanging the hydroxyl protons with tritium. The tritium was quantified by combustion to tritiated water followed by scintillation counting. The number of hydroxyl groups on a leached and presumably hydroxylated Pyrex glass capillary column was found to be about 2.8 groups per square nanometer. This value was slightly less than the 4.6 groups per square nanometer that is generally accepted for a fully hydroxylated porous silica. Dehydroxylation of the same glass by heating at 600°C left only 0.4 groups per square nanometer while an untreated fused silica had only 0.2 groups per square nanometer.
Similar content being viewed by others
References
K. K. Unger, “Porous Silica — Its Properties and Use as a Support in Column Liquid Chromatography”, Elsevier, Amsterdam, Oxford, New York, 1979, and references cited therein.
R. K. Iler, “The Chemistry of Silica — Solubility, Polymerization. Colloid and Surface Properties, and Biochemistry”, John Wiley & Sons, Inc., New York, 1979, and references cited therein.
B. W. Wright, M. L. Lee, S. W. Graham, L. V. Phillips, D. M. Hercules, J. Chromatogr.199, 355 (1980).
M. L. Hair, J. Non-Cryst. Solid19, 299 (1975).
B. W. Wright, M. L. Lee, unpublished results.
R. P. W. Scott, J. Chromatogr. Sci.18, 297 (1980).
H. C. Mattraw, C. F. Pachucki, L. M. Dorfman, J Chem. Phys.20, 926 (1952).
L. M. Dorfman, H. C. Mattraw, J. Phys. Chem.57, 723 (1953).
Z. Suprynowicz, A. Gorgol, J. Wojcik, J. Chromatogr148, 151 (1978).
J. H. DeBoer, J. M. Vleeskens K. Ned. Akad. Wet. Proc. Ser.B, 60, 23, 45, 54 (1957);61, 2, 85 (1958).
L. T. Zhuravlev, A. V. Kiselev, Russ. J. Phys. Chem.39, 236 (1965).
Author information
Authors and Affiliations
Additional information
Dedicated to Dr. Leslie S. Ettre for his 60th birthday.
Rights and permissions
About this article
Cite this article
Wright, B.W., Peaden, P.A., Lee, M.L. et al. Determination of surface hydroxyl concentration on glass and fused silica capillary columns. Chromatographia 15, 584–586 (1982). https://doi.org/10.1007/BF02280379
Issue Date:
DOI: https://doi.org/10.1007/BF02280379