Abstract
An on-line, post-column, extraction system suitable for use with miniaturized HPLC (0.7–1 mm i.d. columns) is described. The system consists of a 30° angle segmenting tee, a PTFE extraction coil of variable length and a membrane phase separator. The internal volume of the phase separator is less than 1 μl. The total variance contribution of the system is between 1 and 6 sec2.
The determination of the anti-cancer drugs VP 16 and VM 26 in urine by means of reversed-phase HPLC and fluorescence detection, using both on-column preconcentration and the present miniaturized post-column extraction system is presented as an application.
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References
R. P. W. Scott J. Chromatogr. Sci.18, 490 (1980).
D. Ishii, K. Asai, K. Hibi, J. Kookuchi, M. Nagoya, J. Chromatogr.144, 157 (1977).
R. W. Frei, Chromatographia15, 161 (1982).
J. A. Apffel, U. A. Th. Brinkman, R. W. Frei, Chromatographia17, 125 (1983).
B. Karlberg, P. Thelander, Anal. Chim. Acta, 98 (1978).
J. F. Lawrence, U. A. Th. Brinkman R. W. Frei J. Chromatogr.171, 73 (1979).
A. H. M. T. Scholten, U. A. Th. Brinkman, R. W. Frei, Anal. Chem. 54, 1982).
J. C. Gfeller, G. Frey, R. W. Frei, J. Chromatogr.142, 271 (1977).
L. Nord, B. Karlberg, Anal. Chim. Acta118, 285 (1980).
K. Ogata, K. Tageuchi, T. Imanari, Anal. Chem.54, 127 (1982).
T. Imasaki, T. Horada, N. Ishibashi, Anal. Chim. Acta129, 195 (1981).
J. Kawase, Anal. Chem.52, 2124 (1980).
R. W. Frei, U. A. Th. Brinkman, TrAC,1, 48 (1980).
H. P. M. van Vliet, Th. C. Bootsman R. W. Frei, U. A. Th. Brinkman, J. Chromatogr.185, 483 (1979).
C. E. Werkhoven-Goewie, C. de Ruiter, U. A. Th. Brinkman, R. W. Frei, G. J. de Jong, C. J. Little, O. Stahel, J. Chromatogr.255, 79 (1983).
W. Th. Kok, U. A. Th. Brinkman, R. W. Frei, H. B. Hanekamp, F. Nooitgedacht, H. Poppe, J. Chromatogr.237, 357 (1982).
C. E. Werkhoven-Goewie, R. W. Frei, U. A. Th. Brinkman, C. de Ruiter, J. de Vries, J. Chromatogr. in press.
J. F. K. Huber, J. A. R. J. Hulsman, C. A. M. Meijers, J. Chromatogr.62, 79 (1971).
R. Tijssen, Anal. Chim. Acta114, 71 (1980).
P. Vouros, E. P. Lankmayr, M. J. Hayes, B. L. Karger, J. M. McGuire, J. Chromatogr.251, 175 (1982).
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Apffel, J.A., Brinkman, U.A.T. & Frei, R.W. Design and application of a post-column extraction system compatible with miniaturized liquid chromatography. Chromatographia 18, 5–10 (1984). https://doi.org/10.1007/BF02279454
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DOI: https://doi.org/10.1007/BF02279454