Summary
A procedure for the simultaneous quantitative determination of amiloride hydrochloride and hydrochlorothiazide by high-performance liquid chromatography is proposed. A reversed-phase LiChrosorb C8 stationary phase is used. The eluent consisted of an acetonitrile/0.1M phosphate buffer pH3 (15∶85) mixture, containing 50mM propylamine hydrochloride. In this system amiloride hydrochloride, a basic drug, eluted with a acceptable asymmetry factor (Asf=2.1). A simple extraction procedure with methanol is used. Relative standard deviations of 0.87% and 1.6% were obtained for amiloride hydrochloride and hydrochlorothiazide respectively. Chlorothiazide, a thiazide diuretic, is a suitable internal standard. Furthermore the method is also specific for other thiazide diuretics, potassium-sparing diuretics and loop diuretics and for the respective hydrolysis productes of both drugs. Analysis time is reduced to a minimum; the chromatographic separation is complete within 6 minutes.
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De Croo, F., Van den Bossche, W. & De Moerloose, P. Simultaneous quantitative determination of amiloride hydrochloride and hydrochlorothiazide in tablets by high-performance liquid chromatography. Chromatographia 20, 477–481 (1985). https://doi.org/10.1007/BF02344789
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DOI: https://doi.org/10.1007/BF02344789