Skip to main content
SpringerLink
Log in

Effects of eluent composition, ion-pair reagent and temperature on the separation of histones by high performance liquid chromatography

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

The influence of the eluent composition, ion-pair concentration, and temperature on the fractionation of chromosomal proteins by reversed-phase high performance liquid chromatography (HPLC) has been investigated. Based on this examination methods for the troublesome separation of histones H1o/H1b, H2A/H4, and H5.1/H5.2 have been developed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J. D. McGhee, G. Felsenfeld, Ann. Rev. Biochem.49, 1115 (1980).

    Google Scholar 

  2. R. D. Kornberg, Ann. Rev. Biochem.46, 931 (1977).

    Google Scholar 

  3. G. Felsenfeld, Nature (London)271, 115 (1978).

    Google Scholar 

  4. R. Reeves, Biochim. Biophys. Acta782, 343 (1984).

    Google Scholar 

  5. U. Certa, G. von Ehrenstein, Anal. Biochem.118, 147 (1981).

    Google Scholar 

  6. L. R. Gurley, J. G. Valdez, D. A. Prentice, W. D. Spall, Anal. Biochem.129, 132 (1983).

    Google Scholar 

  7. H. Lindner, W. Helliger, B. Puschendorf, J. Chromatogr.357, 301 (1986).

    Google Scholar 

  8. H. Lindner, W. Hellinger, B. Puschendorf, Anal. Biochem.158, 424 (1986).

    Google Scholar 

  9. M. C. McCroskey, V. E. Groppi, J. D. Pearson, Anal. Biochem.163, 427 (1987).

    Google Scholar 

  10. W. Helliger, H. Lindner, S. Hauptlorenz, B. Puschendorf, Biochem. J.255, 23 (1988).

    Google Scholar 

  11. H. Lindner, W. Helliger, B. Puschendorf, J. Chromatogr.450, 309 (1988).

    Google Scholar 

  12. H. Lindner, J. Wesierska-Gadek, W. Helliger, B. Puschendorf, G. Sauermann, J. Chromatogr.472, 243 (1989).

    Google Scholar 

  13. H. Lindner, W. Helliger, B. Puschendorf, Biochem. J.269, 359 (1990).

    Google Scholar 

  14. I. Isenberg, Ann. Rev. Biochem.48, 159 (1979).

    Google Scholar 

  15. L. R. Gurley, D. A. Prentice, J. G. Valdez, W. D. Spall, Anal. Biochem.131, 465 (1983).

    Google Scholar 

  16. L. R. Gurley, J. A. D'Anna, M. Blumenfeld, J. G. Valdez, R. J. Sebring, P. R. Donahue, D. A. Prentice, W. D. Spall, J. Chromatogr.297, 147 (1984).

    Google Scholar 

  17. J. W. Huber, C. L. Seaver, J. Liq. Chromatogr.10, 2337 (1987).

    Google Scholar 

  18. M. J. O'Hare, M. W. Cap, E. C. Nice, N. H. C. Cooke, B. G. Archer, Anal. Biochem.126, 17 (1982).

    Google Scholar 

  19. E. Tomlinson, “Handbook of HPLC For The Separation of Amino Acids, Peptides, and Proteins,”, Vol. I, CRC Press, Boca Raton, Florida, 1987; p. 135.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Medizinische Chemie und Biochemie, Universität Innsbruck, Fritz-Pregl-Str. 3, A-6020, Innsbruck, Austria

    H. Lindner & W. Helliger

Authors
  1. H. Lindner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Helliger
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to Prof. Dr. Dr. h. c. Erika Cremer on the occasion of her 90th birthday.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lindner, H., Helliger, W. Effects of eluent composition, ion-pair reagent and temperature on the separation of histones by high performance liquid chromatography. Chromatographia 30, 518–522 (1990). https://doi.org/10.1007/BF02269797

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02269797

Key Words

Search

Navigation