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Identification and determination of fumonisin FB1 and FB2 in corn and corn products by high-performance liquid chromatography-electrospray-ionization tandem mass spectrometry (HPLC-ESI-MS-MS)

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Summary

A new method has been developed for qualitative and quantitative analysis of fumonisin FB1 and FB2 in corn and corn products. After extractive sample preparation followed by solid phase extraction using a strong anionexchanger, highest selectivity and sensitivity was achieved by high-performance liquid chromatography-electrospray-ionization, tandem mass spectrometry (HPLC-ESI-MS-MS) analysis combined with selected reaction monitoring (SRM). With deuterated fumonisin FB1-d6 as internal standard the routine quantitation limit for FB1 was set at 400 pg (0.8 ng g−1 sample) with a signal-to-noise ratio of 10∶1. Almost all corn grit and corn meal samples under study were found to be contaminated with fumonisin FB1 and FB2 in concentrations ranging from 1 to 200 ng g−1. Lower fumonisin levels were found in cornflakes, popcorn and baby food, while no fumonisins were detected in canned sweet corn.

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Authors and Affiliations

  1. Lehrstuhl für Lebensmittelchemie, Universität Würzburg, Am Hubland, 97074, Würzburg, Germany

    Z. Lukacs, S. Schaper, M. Herderich, P. Schreier & H. -U. Humpf

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  1. Z. Lukacs
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  2. S. Schaper
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  3. M. Herderich
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  4. P. Schreier
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  5. H. -U. Humpf
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Lukacs, Z., Schaper, S., Herderich, M. et al. Identification and determination of fumonisin FB1 and FB2 in corn and corn products by high-performance liquid chromatography-electrospray-ionization tandem mass spectrometry (HPLC-ESI-MS-MS). Chromatographia 43, 124–128 (1996). https://doi.org/10.1007/BF02292939

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  • DOI: https://doi.org/10.1007/BF02292939

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