Summary
A new high-performance liquid chromatographic method with column-switching has been developed for the simultaneous determination of parathion and its metabolites such asp-nitrophenol and paraoxon in serum. Serum samples were injected onto a precolumn packed with LiChroprep RP-8 after simple dilution with 20% phosphoric acid. Polar plasma components were washed with 0.02 M phosphate buffer (pH 3.0). After valve switching, the concentrated compounds were eluted in back-flush mode and separated on a Ultracarb ODS 30 column with a gradient of acetonitrile −0.01 M phosphate buffer (pH 3.0) as mobile phase. The method showed excellent precision, accuracy and speed with detection limit of 0.1 μg mL−1. Total analysis time per sample was <40 min and coefficients of variation for intra-and inter-assay were <4.5%.
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Lee, H.S., Kim, K., Kim, J.H. et al. Simultaneous determination of parathion and metabolites in serum by HPLC with column switching. Chromatographia 44, 473–476 (1997). https://doi.org/10.1007/BF02466739
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DOI: https://doi.org/10.1007/BF02466739