Summary
A liquid-solid extraction and purification procedure (LSE) was developed to identify and quantify polyphenols in the leaf tissue ofMyrtus communis L. Identification and quantitation of individual compounds was performed using HPTLC, HPLC-DAD and HPLC-MS analysis. Leaves ofMyrtus communis L. contain small amounts of phenolic acids (caffeic, ellagic and gallic acids) and quercetin derivatives (quercetin 3-O-galactoside and quercetin 3-O-rhamnoside), whereas catechin derivatives (epigallocatechin, epigallocatechin 3-O-gallate, epicatechin 3-O-gallate) and myricetin derivatives (myricetin 3-O-galactoside, myricetin 3-O-rhamnoside) are present in large amounts. This is the first report on the occurrence of galloyl-derivatives of catechin and gallo-catechin inMyrtus communis L. leaves.
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H. I. El Sissi, M. A. I. El Ansary, Planta Med.67, 41 (1967).
J. Hinou, N. Lakkas, S. Phillianos, Plantes Med. Phytother.22, 98 (1988).
H. A. A. Twaij, H. M. S. Ali, A. M. Al-Zohyri, J. Biol. Sci. Res.19, 29 (1988).
M. Ikram, I. Haq, Fitoterapia51, 231 (1980).
R. Gucci, R. Massai, S. Casano, E. Gravano, M. Lucchesini, in G. M. J. Mohren, K. Kramer, S. Sabate, Eds, Impacts of Global Change on Tree Physiology and Forest Ecosystems, Kluwer Academic Publ., Dordrecht, The Netherlands, 1997, p. 225.
J. D. Tenhunen, W. Beyshlag, O. L. Lange, P. C. Harley, in J. D. Tenhunen, F. M. Catarino, O. L. Lange, W. C. Oeschel, Eds., Plant Responses to Stresses. Functional Analysis in Mediterrean Ecosystems, Springer-Verlag, New York, 1987, p. 304.
D. Heimler, A. Pieroni, M. Tattini, A. Cimato, Chromatographia33, 369 (1992).
A. Baldi, A. Romani, S. Tatti, N. Mulinacci, F. F. Vincieri, in R. Brouillard, M. Jay, A. Scalbert, Eds., Polyphenols 94, INRA Editions, Paris, 1995, p. 269.
A. Romani, A. Baldi, N. Mulinacci, F. F. Vincieri, M. Tattini, Chromatographia42, 571 (1996).
J. B. Harborne, The Flavonoids, Adavances in Research since 1986, Chapman & Hall, London, 1994.
M. Vanhaelen, M. Vanhaelen-Fastré, Planta Medica39, 164 (1980).
A. M. Diaz, A. Abeger, Fitoterapia58, 167 (1987).
M. I. Joseph, N. Pichon-Prum, J. Raynaud, Pharmazie42, 142 (1987).
N. Pichon, M. I. Joseph, J. Raynaud, Plantes Med. Phytother.26, 86 (1993).
D. R. Hoagland, D. I. Arnon, Calif, Exp. Sta. Circ.347, (1950).
M. Kim, N. Hagiwara, S. J. Smith, T. Yamamoto, T. Yamane, T. Takahashi, in C. T. Ho, T. Osawa, M. T. Huatig, R. T. Rosen, Eds., Food Phytochemicals for Cancer Prevention II. Teas, Spices and Herbs, Amer. Chem. Soc. Ed., Washington DC, 1984, p. 51.
J. Hinou, C. Demetzous, Q. Harvale, Fitoterapia60, 94 (1989).
C. T. Ho, T. Ferraro, Q. Chen, R. T. Rosen, M. T. Huang in C. T. Ho, T. Osawa, M. T. Huang, R. T. Rosen, Eds., Food Phytochemicals for Cancer Prevention II. Teas, Spices and Herbs, Amer. Chem. Soc. Ed., Washington DC, 1994, p. 51.
R. R. Northup, Z Yu, R. A. Dahlgren, K. A. Vogt, Nature377, 227 (1996).
R. Gucci, M. Tattini, Horticoltural Review21, 177 (1997).
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Romani, A., Pinelli, P., Mulinacci, N. et al. Identification and quantitation of polyphenols in leaves ofMyrtus communis L.. Chromatographia 49, 17–20 (1999). https://doi.org/10.1007/BF02467181
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DOI: https://doi.org/10.1007/BF02467181