Crystallization and preliminary X-ray crystallographic analysis of archaeal O⁶-methylguanine–DNA methyltransferase

Crystals of archaeal O⁶-methylguanine–DNA methyltransferase (MGMT) from hyperthermophilic archaeon Pyrococcus kodakaraensis strain KOD1 have been grown at room temperature using polyethylene glycol as a precipitant. The diffraction pattern of the crystal extends to 2.0 Å resolution at room temperature upon exposure to Cu K radiation. The crystal belongs to the space group P2₁2₁2₁ with unit-cell dimensions of a = 52.8, b = 86.6 and c = 39.9 Å. The presence of one molecule per asymmetric unit gives a crystal volume per protein mass (V_m) of 2.3 ų Da⁻¹ and a solvent content of 48% by volume. A full set of X-ray diffraction data was collected to 2.0 Å Bragg spacings from the native crystal.