Elsevier

FEBS Letters

Volume 329, Issues 1–2, 23 August 1993, Pages 139-143
FEBS Letters

Research letters
Human insulin gene enhancer-binding proteins in pancreatic α and β cell lines

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Abstract

Electrophoretic mobility shift assays were performed using oligonucleotides corresponding to known protein binding sites within the human insulin gene enhancer and nuclear extracts from mouse pancreatic α and β cell lines. The results demonstrate that a previously described factor, IUF-1, binds to three sites at −82 (the CT1 box), −215 (the CT2 box), and −319 (the CT3 box) in the human insulin gene enhancer. IUF-1 was present only in β but not in α cells, while all other DNA-binding proteins were present in both cell lines. IUF-1 may therefore be an important determinant of insulin gene β cell-specific expression.

Keywords

Transcription
Gene expression
IUF-1
Islets of Langerhaus

Abbreviations

CAT, chloramphenicol acetyl transferase
HLH, helix loop helix
NRE, negative regulatory element.

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1

Present address: Department of Biochemistry and Molecular Biology, University of Manchester, Stopford Building, Oxford Road, Manchester, M 13 9PT, UK.