Elsevier

Journal of Chromatography A

Volume 104, Issue 1, 29 January 1975, Pages 99-104
Journal of Chromatography A

Separation of dehydrogenases on polyaminomethyl-styrene

https://doi.org/10.1016/S0021-9673(01)85492-3Get rights and content

Abstract

The binding of dehydrogenases, especially alcohol dehydrogenase, and other proteins to several ion exchangers and hydrophobic polymers was investigated. Quantitative parameters for the stability of the polymer—protein complexes (obtained from double reciprocal plots) indicate a high but different affinity of many proteins for polyaminomethylstyrene. The chromatography of a mixture of five dehydrogenases and human serum albumin on polyaminomethylstyrene is described.

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